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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0072

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Characterization method

sugar utilization assay,NMR,sequence homology analysis

Genomic accession number

APRA01000009

Nucelotide position range

555847-581577

Substrate

capsule polysaccharide

Loci

wzc-wzb-wza-gna-psaA-psaB-psaC-psaD-psaE-psaF-wzx-kpsS2-wzy-gtr5-itrA3-galU-ugd-gpi-gne1-pgm

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- F917_03638 ENW48380.1 0 - 1662 (-) APRA01000009.1:555847-557509 -
- F917_03639 ENW48381.1 2041 - 3412 (+) APRA01000009.1:557888-559259 -
- F917_03640 ENW48382.1 3455 - 4472 (-) APRA01000009.1:559302-560319 -
- F917_03641 ENW48383.1 4464 - 6135 (-) APRA01000009.1:560311-561982 -
- F917_03642 ENW48384.1 6131 - 7394 (-) APRA01000009.1:561978-563241 -
- F917_03643 ENW48385.1 7509 - 8385 (-) APRA01000009.1:563356-564232 -
- F917_03644 ENW48386.1 8409 - 9030 (-) APRA01000009.1:564256-564877 -
- F917_03645 ENW48387.1 9042 - 9885 (-) APRA01000009.1:564889-565732 -
- F917_03646 ENW48388.1 9888 - 11226 (-) APRA01000009.1:565735-567073 -
- F917_03647 ENW48389.1 11222 - 12653 (-) APRA01000009.1:567069-568500 -
- F917_03648 ENW48390.1 12630 - 13884 (-) APRA01000009.1:568477-569731 -
- F917_03649 ENW48391.1 13880 - 14933 (-) APRA01000009.1:569727-570780 -
- F917_03650 ENW48392.1 14934 - 15450 (-) APRA01000009.1:570781-571297 -
- F917_03651 ENW48393.1 15443 - 16490 (-) APRA01000009.1:571290-572337 -
- F917_03652 ENW48394.1 16544 - 17237 (-) APRA01000009.1:572391-573084 -
- F917_03653 ENW48395.1 17239 - 18400 (-) APRA01000009.1:573086-574247 -
- F917_03654 ENW48396.1 18401 - 19400 (-) APRA01000009.1:574248-575247 -
- F917_03655 ENW48397.1 19446 - 20721 (-) APRA01000009.1:575293-576568 -
- F917_03656 ENW48398.1 21076 - 22177 (+) APRA01000009.1:576923-578024 -
- F917_03657 ENW48399.1 22181 - 22610 (+) APRA01000009.1:578028-578457 -
- F917_03658 ENW48400.1 22629 - 24816 (+) APRA01000009.1:578476-580663 -
- F917_03659 ENW48401.1 25008 - 25731 (+) APRA01000009.1:580855-581578 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1662 (-) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes
- 2042 - 3412 (+) other Yes
- 3456 - 4472 (-) other Yes
- 4465 - 6135 (-) other Yes
- 6132 - 7394 (-) other Yes
- 7510 - 8385 (-) other Yes
- 8410 - 9030 (-) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 9043 - 9885 (-) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 9889 - 11226 (-) other Yes
- 11223 - 12653 (-) other Yes
- 12631 - 13884 (-) other Yes
- 13881 - 14933 (-) other Yes
- 14935 - 15450 (-) other Yes
- 15444 - 16490 (-) CAZyme: GT0 Yes
- 16545 - 17237 (-) other Yes
- 17240 - 18400 (-) STP: STP|Aminotran_1_2 Yes
- 18402 - 19400 (-) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 19447 - 20721 (-) other Yes
- 21077 - 22177 (+) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 22182 - 22610 (+) other Yes
- 22630 - 24816 (+) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 25009 - 25731 (+) CDS No

PUL ID

PUL0072

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Title

Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster.

Author

Kenyon JJ, Arbatsky NP, Sweeney EL, Shashkov AS, Shneider MM, Popova AV, Hall RM, Knirel YA

Abstract

A new capsular polysaccharide (CPS) biosynthesis gene cluster, KL16, was found in the genome sequence of a clinical Acinetobacter baumannii ST25 isolate, D4. The variable part of KL16 contains a module of genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (5,7-di-N-acetylpseudaminic acid, Pse5Ac7Ac), a gene encoding ItrA3 that initiates the CPS synthesis with d-GlcpNAc, and two glycosyltransferase (Gtr) genes. The K16 CPS was studied by sugar analysis and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy, and shown to be built up of linear trisaccharide repeats containing d-galactose (d-Gal), N-acetyl-d-glucosamine (d-GlcNAc), and Pse5Ac7Ac. The d-Galp residue is linked to the d-GlcpNAc initiating sugar via a beta-(1 --> 3) linkage evidently formed by a Gtr5 variant, Gtr5(K16), encoded in KL16. This reveals an altered or relaxed substrate specificity of this variant as the majority of Gtr5-type glycosyltransferases have previously been shown to form a beta-d-Galp-(1 --> 3)-d-GalpNAc linkage. The beta-Psep5Ac7Ac-(2 --> 4)-d-Galp linkage is predicted to be formed by the other glycosyltransferase, Gtr37, which does not match members of any known glycosyltransferase family.