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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0073

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Characterization method

sugar utilization assay,NMR,sequence homology analysis

Genomic accession number

APOF01000020

Nucelotide position range

103755-129611

Substrate

capsule polysaccharide

Loci

wzc-wzb-wza-gna-psaA-psaB-psaC-psaG-psaH-psaF-wzx-kpsS2-wzy-gtr5-itrA3-galU-ugd-gpi-gne1-pgm

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- F996_03576 ENU11478.1 0 - 1662 (-) APOF01000020.1:103755-105417 -
- F996_03577 ENU11479.1 2041 - 3412 (+) APOF01000020.1:105796-107167 -
- F996_03578 ENU11480.1 3455 - 4472 (-) APOF01000020.1:107210-108227 -
- F996_03579 ENU11481.1 4464 - 6135 (-) APOF01000020.1:108219-109890 -
- F996_03580 ENU11482.1 6131 - 7394 (-) APOF01000020.1:109886-111149 -
- F996_03581 ENU11483.1 7509 - 8385 (-) APOF01000020.1:111264-112140 -
- F996_03582 ENU11484.1 8409 - 9030 (-) APOF01000020.1:112164-112785 -
- F996_03583 ENU11485.1 9042 - 9873 (-) APOF01000020.1:112797-113628 -
- F996_03584 ENU11486.1 9945 - 10926 (-) APOF01000020.1:113700-114681 -
- F996_03585 ENU11487.1 10971 - 12408 (-) APOF01000020.1:114726-116163 -
- F996_03586 ENU11488.1 12385 - 13639 (-) APOF01000020.1:116140-117394 -
- F996_03587 ENU11489.1 13635 - 14676 (-) APOF01000020.1:117390-118431 -
- F996_03588 ENU11490.1 14702 - 15602 (-) APOF01000020.1:118457-119357 -
- F996_03589 ENU11491.1 15594 - 16677 (-) APOF01000020.1:119349-120432 -
- F996_03590 ENU11492.1 16673 - 17363 (-) APOF01000020.1:120428-121118 -
- F996_03591 ENU11493.1 17365 - 18526 (-) APOF01000020.1:121120-122281 -
- F996_03592 ENU11494.1 18527 - 19526 (-) APOF01000020.1:122282-123281 -
- F996_03593 ENU11495.1 19572 - 20847 (-) APOF01000020.1:123327-124602 -
- F996_03594 ENU11496.1 21202 - 22303 (+) APOF01000020.1:124957-126058 -
- F996_03595 ENU11497.1 22307 - 22736 (+) APOF01000020.1:126062-126491 -
- F996_03596 ENU11498.1 22755 - 24942 (+) APOF01000020.1:126510-128697 -
- F996_03597 ENU11499.1 25134 - 25857 (+) APOF01000020.1:128889-129612 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1662 (-) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes
- 2042 - 3412 (+) other Yes
- 3456 - 4472 (-) other Yes
- 4465 - 6135 (-) other Yes
- 6132 - 7394 (-) other Yes
- 7510 - 8385 (-) other Yes
- 8410 - 9030 (-) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 9043 - 9873 (-) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 9946 - 10926 (-) other Yes
- 10972 - 12408 (-) other Yes
- 12386 - 13639 (-) other Yes
- 13636 - 14676 (-) other Yes
- 14703 - 15602 (-) other Yes
- 15595 - 16677 (-) other Yes
- 16674 - 17363 (-) other Yes
- 17366 - 18526 (-) STP: STP|Aminotran_1_2 Yes
- 18528 - 19526 (-) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 19573 - 20847 (-) other Yes
- 21203 - 22303 (+) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 22308 - 22736 (+) other Yes
- 22756 - 24942 (+) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 25135 - 25857 (+) CDS No

PUL ID

PUL0073

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Title

Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster.

Author

Kenyon JJ, Arbatsky NP, Sweeney EL, Shashkov AS, Shneider MM, Popova AV, Hall RM, Knirel YA

Abstract

A new capsular polysaccharide (CPS) biosynthesis gene cluster, KL16, was found in the genome sequence of a clinical Acinetobacter baumannii ST25 isolate, D4. The variable part of KL16 contains a module of genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (5,7-di-N-acetylpseudaminic acid, Pse5Ac7Ac), a gene encoding ItrA3 that initiates the CPS synthesis with d-GlcpNAc, and two glycosyltransferase (Gtr) genes. The K16 CPS was studied by sugar analysis and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy, and shown to be built up of linear trisaccharide repeats containing d-galactose (d-Gal), N-acetyl-d-glucosamine (d-GlcNAc), and Pse5Ac7Ac. The d-Galp residue is linked to the d-GlcpNAc initiating sugar via a beta-(1 --> 3) linkage evidently formed by a Gtr5 variant, Gtr5(K16), encoded in KL16. This reveals an altered or relaxed substrate specificity of this variant as the majority of Gtr5-type glycosyltransferases have previously been shown to form a beta-d-Galp-(1 --> 3)-d-GalpNAc linkage. The beta-Psep5Ac7Ac-(2 --> 4)-d-Galp linkage is predicted to be formed by the other glycosyltransferase, Gtr37, which does not match members of any known glycosyltransferase family.