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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0074

PubMed

31396168, Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.

Characterization method

sequence homology analysis

Genomic accession number

GL636865.1

Nucelotide position range

1623-31927

Substrate

capsule polysaccharide

Loci

wzc- wzb- wza- gfndA+ rmlB+ rmlD+ rmlA+ rmlC+ wzx+ wafX+ wzy+ wafY+ wafZ+ wagA+ gnaB+ wagB+weeH+ galU+ ugd+ gpi+ cgmA+ pgm- lldp

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - GL636865_1 3 - 722 (-) GL636865.1:1626-2345 -
- - GL636865_2 664 - 1068 (-) GL636865.1:2287-2691 -
- - GL636865_3 1065 - 1817 (-) GL636865.1:2688-3440 -
- - GL636865_4 1837 - 3591 (-) GL636865.1:3460-5214 -
- - GL636865_5 3879 - 5249 (+) GL636865.1:5502-6872 -
- - GL636865_6 5277 - 7118 (-) GL636865.1:6900-8741 -
- - GL636865_7 7293 - 8810 (-) GL636865.1:8916-10433 -
- - GL636865_8 8780 - 8923 (-) GL636865.1:10403-10546 -
- - GL636865_9 8920 - 10182 (-) GL636865.1:10543-11805 -
- - GL636865_10 10298 - 11173 (-) GL636865.1:11921-12796 -
- - GL636865_11 11197 - 11811 (-) GL636865.1:12820-13434 -
- - GL636865_12 12101 - 12625 (-) GL636865.1:13724-14248 -
- - GL636865_13 12585 - 13133 (-) GL636865.1:14208-14756 -
- - GL636865_14 13134 - 14270 (-) GL636865.1:14757-15893 -
- - GL636865_15 14263 - 14859 (-) GL636865.1:15886-16482 -
- - GL636865_16 14856 - 15656 (-) GL636865.1:16479-17279 -
- - GL636865_17 15635 - 16738 (-) GL636865.1:17258-18361 -
- - GL636865_18 16725 - 17480 (-) GL636865.1:18348-19103 -
- - GL636865_19 17794 - 18078 (+) GL636865.1:19417-19701 -
- - GL636865_20 18153 - 18275 (-) GL636865.1:19776-19898 -
- - GL636865_21 18539 - 19231 (-) GL636865.1:20162-20854 -
- - GL636865_22 19209 - 19448 (-) GL636865.1:20832-21071 -
- - GL636865_23 19602 - 20870 (-) GL636865.1:21225-22493 -
- - GL636865_24 21140 - 21706 (-) GL636865.1:22763-23329 -
- - GL636865_25 21764 - 22657 (-) GL636865.1:23387-24280 -
- - GL636865_26 22657 - 23061 (-) GL636865.1:24280-24684 -
- - GL636865_27 23431 - 23529 (-) GL636865.1:25054-25152 -
- - GL636865_28 23546 - 23785 (-) GL636865.1:25169-25408 -
- - GL636865_29 23828 - 24586 (-) GL636865.1:25451-26209 -
- - GL636865_30 24609 - 25574 (-) GL636865.1:26232-27197 -
- - GL636865_31 25531 - 25764 (-) GL636865.1:27154-27387 -
- - GL636865_32 25736 - 25831 (-) GL636865.1:27359-27454 -
- - GL636865_33 25955 - 27136 (+) GL636865.1:27578-28759 -
- - GL636865_34 27114 - 27566 (+) GL636865.1:28737-29189 -
- - GL636865_35 27588 - 29783 (+) GL636865.1:29211-31406 -
- - GL636865_36 29970 - 30146 (+) GL636865.1:31593-31769 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 3 - 722 (-) CDS No
- 664 - 1068 (-) CDS No
- 1065 - 1817 (-) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0039384 No
- 1837 - 3591 (-) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes
- 3879 - 5249 (+) other Yes
- 5277 - 7118 (-) other Yes
- 7293 - 8810 (-) other Yes
- 8780 - 8923 (-) other Yes
- 8920 - 10182 (-) other Yes
- 10298 - 11173 (-) other Yes
- 11197 - 11811 (-) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 12101 - 12625 (-) other Yes
- 12585 - 13133 (-) other Yes
- 13134 - 14270 (-) other Yes
- 14263 - 14859 (-) other Yes
- 14856 - 15656 (-) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 15635 - 16738 (-) CAZyme: GT4 Yes
- 16725 - 17480 (-) other Yes
- 17794 - 18078 (+) other Yes
- 18153 - 18275 (-) other Yes
- 18539 - 19231 (-) other Yes
- 19209 - 19448 (-) other Yes
- 19602 - 20870 (-) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 21140 - 21706 (-) other Yes
- 21764 - 22657 (-) other Yes
- 22657 - 23061 (-) other Yes
- 23431 - 23529 (-) other Yes
- 23546 - 23785 (-) other Yes
- 23828 - 24586 (-) other Yes
- 24609 - 25574 (-) other Yes
- 25531 - 25764 (-) other Yes
- 25736 - 25831 (-) other Yes
- 25955 - 27136 (+) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 27114 - 27566 (+) other Yes
- 27588 - 29783 (+) TC: gnl|TC-DB|Q45409|8.A.3.3.3 Yes
- 29970 - 30146 (+) CDS No

PUL ID

PUL0074

PubMed

31396168, Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.

Title

Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118.

Author

Ramirez MS, Penwell WF, Traglia GM, Zimbler DL, Gaddy JA, Nikolaidis N, Arivett BA, Adams MD, Bonomo RA, Actis LA, Tolmasky ME

Abstract

Acinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain A118 a convenient model for genetic studies of A. baumannii. To identify potential virulence factors, its complete genome was analyzed and compared to other A. baumannii genomes. A. baumannii A118 includes gene clusters coding for the acinetobactin and baumannoferrin iron acquisition systems. Iron-regulated expression of the BauA outer membrane receptor for ferric-acinetobactin complexes was confirmed as well as the utilization of acinetobactin. A. baumannii A118 also possesses the feoABC genes, which code for the main bacterial ferrous uptake system. The functionality of baumannoferrin was suggested by the ability of A. baumannii A118 culture supernatants to cross feed an indicator BauA-deficient strain plated on iron-limiting media. A. baumannii A118 behaved as non-motile but included the csuA/BABCDE chaperone-usher pilus assembly operon and produced biofilms on polystyrene and glass surfaces. While a known capsular polysaccharide (K) locus was identified, the outer core polysaccharide (OC) locus, which belongs to group B, showed differences with available sequences. Our results show that despite being susceptible to most antibiotics, strain A118 conserves known virulence-related traits enhancing its value as model to study A. baumannii pathogenicity.