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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0075

PubMed

31396168, Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.

Characterization method

sequence homology analysis

Genomic accession number

KC526898.2

Nucelotide position range

537-30586

Substrate

capsule polysaccharide

Loci

wzc- wzb- wza- gfndA+ rmlB+ rmlD+ rmlA+ rmlC+ wzx+ wafX+ wzy+ wafY+ wafZ+ wagA+ gnaB+ wagB+weeH+ galU+ ugd+ gpi+ cgmA+ pgm- lldp

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - AHB32321.1 537 - 2733 (-) KC526898.2:1074-3270 -
wzb - AHB32320.2 2754 - 3183 (-) KC526898.2:3291-3720 -
wza - AHB32319.2 3184 - 4366 (-) KC526898.2:3721-4903 -
gna - AHB32318.1 4489 - 5767 (+) KC526898.2:5026-6304 -
rmlB - AHB32317.1 5789 - 6866 (+) KC526898.2:6326-7403 -
rmlD - AHB32316.2 6882 - 7788 (+) KC526898.2:7419-8325 -
rmlA - AHB32315.1 7787 - 8681 (+) KC526898.2:8324-9218 -
rmlC - AHB32314.1 8738 - 9305 (+) KC526898.2:9275-9842 -
wzx - AHB32313.1 9574 - 10843 (+) KC526898.2:10111-11380 -
gtr154 - AHB32312.1 10996 - 11905 (+) KC526898.2:11533-12442 -
wzy - AHB32311.1 12703 - 13738 (+) KC526898.2:13240-14275 -
gtr27 - AHB32310.2 13775 - 14828 (+) KC526898.2:14312-15365 -
gtr60 - AHB32309.1 14806 - 15607 (+) KC526898.2:15343-16144 -
atr8 - AHB32308.1 15603 - 16200 (+) KC526898.2:16140-16737 -
tle - AHB32307.1 16192 - 17329 (+) KC526898.2:16729-17866 -
gtr29 - AHB32306.1 17329 - 18370 (+) KC526898.2:17866-18907 -
itrA3 - AHB32305.2 18660 - 19275 (+) KC526898.2:19197-19812 -
galU - AHB32304.1 19298 - 20174 (+) KC526898.2:19835-20711 -
ugd - AHB32303.1 20289 - 21552 (+) KC526898.2:20826-22089 -
gpi - AHB32302.2 21548 - 23219 (+) KC526898.2:22085-23756 -
pgt1 - AHB32301.1 23393 - 25235 (+) KC526898.2:23930-25772 -
pgm - AHB32300.2 25261 - 26632 (-) KC526898.2:25798-27169 -
lldP - AHB32299.1 26998 - 28666 (+) KC526898.2:27535-29203 -
lldR - AHB32298.2 28685 - 29438 (+) KC526898.2:29222-29975 -
lldD - AHB32297.1 29434 - 30586 (+) KC526898.2:29971-31123 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 538 - 2733 (-) TC: gnl|TC-DB|Q45409|8.A.3.3.3 Yes
- 2755 - 3183 (-) other Yes
- 3185 - 4366 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 4490 - 5767 (+) other Yes
- 5790 - 6866 (+) other Yes
- 6883 - 7788 (+) other Yes
- 7788 - 8681 (+) other Yes
- 8739 - 9305 (+) other Yes
- 9575 - 10843 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 10997 - 11905 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 12704 - 13738 (+) other Yes
- 13776 - 14828 (+) CAZyme: GT4 Yes
- 14807 - 15607 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 15604 - 16200 (+) other Yes
- 16193 - 17329 (+) other Yes
- 17330 - 18370 (+) other Yes
- 18661 - 19275 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 19299 - 20174 (+) other Yes
- 20290 - 21552 (+) other Yes
- 21549 - 23219 (+) other Yes
- 23394 - 25235 (+) other Yes
- 25262 - 26632 (-) other Yes
- 26999 - 28666 (+) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes
- 28686 - 29438 (+) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0039384 No
- 29435 - 30586 (+) CDS No

PUL ID

PUL0075

PubMed

31396168, Front Microbiol. 2019 Jul 23;10:1599. doi: 10.3389/fmicb.2019.01599. eCollection 2019.

Title

Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118.

Author

Ramirez MS, Penwell WF, Traglia GM, Zimbler DL, Gaddy JA, Nikolaidis N, Arivett BA, Adams MD, Bonomo RA, Actis LA, Tolmasky ME

Abstract

Acinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain A118 a convenient model for genetic studies of A. baumannii. To identify potential virulence factors, its complete genome was analyzed and compared to other A. baumannii genomes. A. baumannii A118 includes gene clusters coding for the acinetobactin and baumannoferrin iron acquisition systems. Iron-regulated expression of the BauA outer membrane receptor for ferric-acinetobactin complexes was confirmed as well as the utilization of acinetobactin. A. baumannii A118 also possesses the feoABC genes, which code for the main bacterial ferrous uptake system. The functionality of baumannoferrin was suggested by the ability of A. baumannii A118 culture supernatants to cross feed an indicator BauA-deficient strain plated on iron-limiting media. A. baumannii A118 behaved as non-motile but included the csuA/BABCDE chaperone-usher pilus assembly operon and produced biofilms on polystyrene and glass surfaces. While a known capsular polysaccharide (K) locus was identified, the outer core polysaccharide (OC) locus, which belongs to group B, showed differences with available sequences. Our results show that despite being susceptible to most antibiotics, strain A118 conserves known virulence-related traits enhancing its value as model to study A. baumannii pathogenicity.