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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0092

PubMed

31275257, Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.

Characterization method

sequence homology analysis

Genomic accession number

CP000139.1

Nucelotide position range

5092673-5110584

Substrate

O-glycan,N-glycan

Loci

BVU_4138-BVU_4147

Species

Bacteroides vulgatus/821

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BVU_4138 ABR41740.1 191 - 2516 (-) CP000139.1:5092864-5095189 -
- BVU_4139 ABR41741.1 2532 - 5100 (-) CP000139.1:5095205-5097773 -
- BVU_4140 ABR41742.1 5126 - 7190 (-) CP000139.1:5097799-5099863 -
- BVU_4141 ABR41743.1 7186 - 7852 (-) CP000139.1:5099859-5100525 -
- BVU_4142 ABR41744.1 7853 - 9860 (-) CP000139.1:5100526-5102533 -
- BVU_4143 ABR41745.1 9863 - 11504 (-) CP000139.1:5102536-5104177 -
- BVU_4144 ABR41746.1 11636 - 13139 (-) CP000139.1:5104309-5105812 -
- BVU_4145 ABR41747.1 13158 - 16434 (-) CP000139.1:5105831-5109107 -
- BVU_4147 ABR41748.1 17019 - 18060 (+) CP000139.1:5109692-5110733 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 5 (-) CDS No
- 192 - 2516 (-) CAZyme: GH20 Yes
- 2533 - 5100 (-) CAZyme: GH2 Yes
- 5127 - 7190 (-) other Yes
- 7187 - 7852 (-) other Yes
- 7854 - 9860 (-) CAZyme: GH20 Yes
- 9864 - 11504 (-) CAZyme: GH33 Yes
- 11637 - 13139 (-) TC: gnl|TC-DB|Q5LEN2|8.A.46.1.4 Yes
- 13159 - 16434 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 17020 - 18060 (+) CDS No

PUL ID

PUL0092

PubMed

31275257, Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.

Title

Investigating Host Microbiota Relationships Through Functional Metagenomics.

Author

Laville E, Perrier J, Bejar N, Maresca M, Esque J, Tauzin AS, Bouhajja E, Leclerc M, Drula E, Henrissat B, Berdah S, Di Pasquale E, Robe P, Potocki-Veronese G

Abstract

The human Intestinal mucus is formed by glycoproteins, the O- and N-linked glycans which constitute a crucial source of carbon for commensal gut bacteria, especially when deprived of dietary glycans of plant origin. In recent years, a dozen carbohydrate-active enzymes from cultivated mucin degraders have been characterized. But yet, considering the fact that uncultured species predominate in the human gut microbiota, these biochemical data are far from exhaustive. In this study, we used functional metagenomics to identify new metabolic pathways in uncultured bacteria involved in harvesting mucin glycans. First, we performed a high-throughput screening of a fosmid metagenomic library constructed from the ileum mucosa microbiota using chromogenic substrates. The screening resulted in the isolation of 124 clones producing activities crucial in the degradation of human O- and N-glycans, namely sialidases, beta-D-N-acetyl-glucosaminidase, beta-D-N-acetyl-galactosaminidase, and/or beta-D-mannosidase. Thirteen of these clones were selected based on their diversified functional profiles and were further analyzed on a secondary screening. This step consisted of lectin binding assays to demonstrate the ability of the clones to degrade human intestinal mucus. In total, the structural modification of several mucin motifs, sialylated mucin ones in particular, was evidenced for nine clones. Sequencing their metagenomic loci highlighted complex catabolic pathways involving the complementary functions of glycan sensing, transport, hydrolysis, deacetylation, and deamination, which were sometimes associated with amino acid metabolism machinery. These loci are assigned to several Bacteroides and Feacalibacterium species highly prevalent and abundant in the gut microbiome and explain the metabolic flexibility of gut bacteria feeding both on dietary and human glycans.