Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0156

PubMed

30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.

Characterization method

RNA-Seq,differential gene expression

Genomic accession number

MSTC01000055.1

Nucelotide position range

620-3443

Substrate

lactose

Loci

BVG98_07735-BVG98_07740

Species

Lactobacillus rhamnosus/47715

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BVG98_07735 ONG00400.1 0 - 1881 (+) MSTC01000055.1:620-2501 -
- BVG98_07740 ONG00401.1 1864 - 2824 (+) MSTC01000055.1:2484-3444 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1881 (+) CAZyme: GH2 No
- 1865 - 2824 (+) CDS No

PUL ID

PUL0156

PubMed

30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.

Title

Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus.

Author

Arnold JW, Simpson JB, Roach J, Bruno-Barcena JM, Azcarate-Peril MA

Abstract

Lactose intolerance, characterized by a decrease in host lactase expression, affects approximately 75% of the world population. Galacto-oligosaccharides (GOS) are prebiotics that have been shown to alleviate symptoms of lactose intolerance and to modulate the intestinal microbiota, promoting the growth of beneficial microorganisms. We hypothesized that mechanisms of GOS utilization by intestinal bacteria are variable, impacting efficacy and response, with differences occurring at the strain level. This study aimed to determine the mechanisms by which human-derived Lactobacillus rhamnosus strains metabolize GOS. Genomic comparisons between strains revealed differences in carbohydrate utilization components, including transporters, enzymes for degradation, and transcriptional regulation, despite a high overall sequence identity (>95%) between strains. Physiological and transcriptomics analyses showed distinct differences in carbohydrate metabolism profiles and GOS utilization between strains. A putative operon responsible for GOS utilization was identified and characterized by genetic disruption of the 6-phospho-beta-galactosidase, which had a critical role in GOS utilization. Our findings highlight the importance of strain-specific bacterial metabolism in the selection of probiotics and synbiotics to alleviate symptoms of gastrointestinal disorders including lactose intolerance.