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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0158

PubMed

30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.

Characterization method

RNA-Seq,differential gene expression

Genomic accession number

NC_013199.1

Nucelotide position range

311240-315872

Substrate

lactose

Loci

Lc705_RS01460-Lc705_RS01475

Species

Lactobacillus rhamnosus/47715

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- LC705_RS01460 WP_005714797.1 0 - 1794 (+) NC_013199.1:311240-313034 -
- LC705_RS01465 WP_014571077.1 1847 - 2717 (+) NC_013199.1:313087-313957 -
- LC705_RS01470 WP_005691321.1 2817 - 3183 (+) NC_013199.1:314057-314423 -
lacG LC705_RS01475 WP_005691323.1 3208 - 4633 (+) NC_013199.1:314448-315873 3.2.1.85

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1794 (+) TC: gnl|TC-DB|P11162|4.A.3.1.1 Yes
- 1848 - 2717 (+) other Yes
- 2818 - 3183 (+) TC: gnl|TC-DB|Q045H4|4.A.3.1.3 Yes
lacG 3209 - 4633 (+) CAZyme: GH1 Yes

PUL ID

PUL0158

PubMed

30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.

Title

Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus.

Author

Arnold JW, Simpson JB, Roach J, Bruno-Barcena JM, Azcarate-Peril MA

Abstract

Lactose intolerance, characterized by a decrease in host lactase expression, affects approximately 75% of the world population. Galacto-oligosaccharides (GOS) are prebiotics that have been shown to alleviate symptoms of lactose intolerance and to modulate the intestinal microbiota, promoting the growth of beneficial microorganisms. We hypothesized that mechanisms of GOS utilization by intestinal bacteria are variable, impacting efficacy and response, with differences occurring at the strain level. This study aimed to determine the mechanisms by which human-derived Lactobacillus rhamnosus strains metabolize GOS. Genomic comparisons between strains revealed differences in carbohydrate utilization components, including transporters, enzymes for degradation, and transcriptional regulation, despite a high overall sequence identity (>95%) between strains. Physiological and transcriptomics analyses showed distinct differences in carbohydrate metabolism profiles and GOS utilization between strains. A putative operon responsible for GOS utilization was identified and characterized by genetic disruption of the 6-phospho-beta-galactosidase, which had a critical role in GOS utilization. Our findings highlight the importance of strain-specific bacterial metabolism in the selection of probiotics and synbiotics to alleviate symptoms of gastrointestinal disorders including lactose intolerance.