dbCAN-PUL

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.

PUL ID	PUL0159
PubMed	30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
Characterization method	RNA-Seq,differential gene expression
Genomic accession number	FM179324.1
Nucelotide position range	40411-43234
Substrate	lactose
Loci	Lc705_p00049-Lc705_p00050
Species	Lactobacillus rhamnosus/47715
Degradation or Biosynthesis	degradation

Gene Name	Locus Tag	Protein ID	Gene Position	GenBank Contig Range	EC Number
ebgA	LC705_p00049	CAR91801.1	0 - 1881 (+)	FM179324.1:40411-42292	-
lacZ	LC705_p00050	CAR91802.1	1864 - 2824 (+)	FM179324.1:42275-43235	-

Cluster number	0
Gene name	Gene position	Gene type	Found by CGCFinder?
ebgA	1 - 1881 (+)	CAZyme: GH2	No
lacZ	1865 - 2824 (+)	CDS	No

PUL ID	PUL0159
PubMed	30332787, Nutrients. 2018 Oct 16;10(10):1517. doi: 10.3390/nu10101517.
Title	Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus.
Author	Arnold JW, Simpson JB, Roach J, Bruno-Barcena JM, Azcarate-Peril MA
Abstract	Lactose intolerance, characterized by a decrease in host lactase expression, affects approximately 75% of the world population. Galacto-oligosaccharides (GOS) are prebiotics that have been shown to alleviate symptoms of lactose intolerance and to modulate the intestinal microbiota, promoting the growth of beneficial microorganisms. We hypothesized that mechanisms of GOS utilization by intestinal bacteria are variable, impacting efficacy and response, with differences occurring at the strain level. This study aimed to determine the mechanisms by which human-derived Lactobacillus rhamnosus strains metabolize GOS. Genomic comparisons between strains revealed differences in carbohydrate utilization components, including transporters, enzymes for degradation, and transcriptional regulation, despite a high overall sequence identity (>95%) between strains. Physiological and transcriptomics analyses showed distinct differences in carbohydrate metabolism profiles and GOS utilization between strains. A putative operon responsible for GOS utilization was identified and characterized by genetic disruption of the 6-phospho-beta-galactosidase, which had a critical role in GOS utilization. Our findings highlight the importance of strain-specific bacterial metabolism in the selection of probiotics and synbiotics to alleviate symptoms of gastrointestinal disorders including lactose intolerance.

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