PUL ID

PUL0160

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry, sequence homology analysis

Genomic accession number

JQNQ01000001.1

Nucelotide position range

1815119-1861584

Substrate

alpha-mannan

Loci

FG27draft_1658-FG27draft_1687

Species

Salegentibacter sp. Hel_I_6/1250278

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - JQNQ01000001_1 1 - 927 (-) JQNQ01000001.1:1815120-1816046 -
- - JQNQ01000001_2 1110 - 2423 (-) JQNQ01000001.1:1816229-1817542 -
- - JQNQ01000001_3 2427 - 4796 (-) JQNQ01000001.1:1817546-1819915 -
- - JQNQ01000001_4 4838 - 7132 (-) JQNQ01000001.1:1819957-1822251 -
- - JQNQ01000001_5 7144 - 8178 (-) JQNQ01000001.1:1822263-1823297 -
- - JQNQ01000001_6 8185 - 8883 (-) JQNQ01000001.1:1823304-1824002 -
- - JQNQ01000001_7 8895 - 10622 (-) JQNQ01000001.1:1824014-1825741 -
- - JQNQ01000001_8 10640 - 11764 (-) JQNQ01000001.1:1825759-1826883 -
- - JQNQ01000001_9 11806 - 13239 (-) JQNQ01000001.1:1826925-1828358 -
- - JQNQ01000001_10 13310 - 13930 (-) JQNQ01000001.1:1828429-1829049 -
- - JQNQ01000001_11 14318 - 15118 (-) JQNQ01000001.1:1829437-1830237 -
- - JQNQ01000001_12 15133 - 16749 (-) JQNQ01000001.1:1830252-1831868 -
- - JQNQ01000001_13 16762 - 19824 (-) JQNQ01000001.1:1831881-1834943 -
- - JQNQ01000001_14 19944 - 22199 (-) JQNQ01000001.1:1835063-1837318 -
- - JQNQ01000001_15 22168 - 23145 (-) JQNQ01000001.1:1837287-1838264 -
- - JQNQ01000001_16 23265 - 25379 (+) JQNQ01000001.1:1838384-1840498 -
- - JQNQ01000001_17 26156 - 26605 (+) JQNQ01000001.1:1841275-1841724 -
- - JQNQ01000001_18 26950 - 28428 (+) JQNQ01000001.1:1842069-1843547 -
- - JQNQ01000001_19 28475 - 29302 (+) JQNQ01000001.1:1843594-1844421 -
- - JQNQ01000001_20 29403 - 29633 (+) JQNQ01000001.1:1844522-1844752 -
- - JQNQ01000001_21 29650 - 29949 (+) JQNQ01000001.1:1844769-1845068 -
- - JQNQ01000001_22 30613 - 31431 (-) JQNQ01000001.1:1845732-1846550 -
- - JQNQ01000001_23 31564 - 33768 (-) JQNQ01000001.1:1846683-1848887 -
- - JQNQ01000001_24 33789 - 34955 (-) JQNQ01000001.1:1848908-1850074 -
- - JQNQ01000001_25 35081 - 36112 (-) JQNQ01000001.1:1850200-1851231 -
- - JQNQ01000001_26 36228 - 36389 (-) JQNQ01000001.1:1851347-1851508 -
- - JQNQ01000001_27 36610 - 36867 (-) JQNQ01000001.1:1851729-1851986 -
- - JQNQ01000001_28 36864 - 37709 (-) JQNQ01000001.1:1851983-1852828 -
- - JQNQ01000001_29 37746 - 38735 (-) JQNQ01000001.1:1852865-1853854 -
- - JQNQ01000001_30 39107 - 40924 (-) JQNQ01000001.1:1854226-1856043 -
- - JQNQ01000001_31 41092 - 42870 (-) JQNQ01000001.1:1856211-1857989 -
- - JQNQ01000001_32 42953 - 45313 (-) JQNQ01000001.1:1858072-1860432 -
- - JQNQ01000001_33 45324 - 46466 (-) JQNQ01000001.1:1860443-1861585 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 927 (-) CDS No
- 1110 - 2423 (-) TC: gnl|TC-DB|A1S5F4|2.A.1.7.10 Yes
- 2427 - 4796 (-) CAZyme: GH92 Yes
- 4838 - 7132 (-) CAZyme: GH92 Yes
- 7144 - 8178 (-) CAZyme: GH43|GH43_34 Yes
- 8185 - 8883 (-) other Yes
- 8895 - 10622 (-) other Yes
- 10640 - 11764 (-) other Yes
- 11806 - 13239 (-) CAZyme: GH125 Yes
- 13310 - 13930 (-) other Yes
- 14318 - 15118 (-) CAZyme: CBM32 Yes
- 15133 - 16749 (-) TC: gnl|TC-DB|Q8A1G2|8.A.46.1.1 Yes
- 16762 - 19824 (-) TC: gnl|TC-DB|G0LBU8|1.B.14.6.11 Yes
- 19944 - 22199 (-) CAZyme: GH92 Yes
- 22168 - 23145 (-) other Yes
- 23265 - 25379 (+) CAZyme: GH92 Yes
- 26156 - 26605 (+) other Yes
- 26950 - 28428 (+) other Yes
- 28475 - 29302 (+) other Yes
- 29403 - 29633 (+) other Yes
- 29650 - 29949 (+) other Yes
- 30613 - 31431 (-) other Yes
- 31564 - 33768 (-) CAZyme: GH92 Yes
- 33789 - 34955 (-) CAZyme: GH76 Yes
- 35081 - 36112 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558 Yes
- 36228 - 36389 (-) other Yes
- 36610 - 36867 (-) other Yes
- 36864 - 37709 (-) other Yes
- 37746 - 38735 (-) other Yes
- 39107 - 40924 (-) CAZyme: GH2 Yes
- 41092 - 42870 (-) other Yes
- 42953 - 45313 (-) CAZyme: GH92 Yes
- 45324 - 46466 (-) TC: gnl|TC-DB|Q8A5Y0|2.A.1.7.16 Yes

PUL ID

PUL0160

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.