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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0162

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis

Genomic accession number

NC_004663.1

Nucelotide position range

5012915-5035451

Substrate

alpha-mannan

Loci

BT_3853-BT_3865

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS19455 WP_008764126.1 0 - 2574 (+) NC_004663.1:5012915-5015489 -
- BT_RS19460 WP_164927913.1 3042 - 6174 (+) NC_004663.1:5015957-5019089 -
- BT_RS19465 WP_008764128.1 6185 - 8117 (+) NC_004663.1:5019100-5021032 -
- BT_RS19470 WP_008764129.1 8145 - 8841 (+) NC_004663.1:5021060-5021756 -
- BT_RS19475 WP_008764130.1 8859 - 10767 (+) NC_004663.1:5021774-5023682 -
- BT_RS19480 WP_011108997.1 10798 - 13066 (+) NC_004663.1:5023713-5025981 -
- BT_RS19485 WP_008760786.1 13104 - 15081 (+) NC_004663.1:5026019-5027996 -
- BT_RS19490 WP_008764132.1 15114 - 16245 (+) NC_004663.1:5028029-5029160 -
- BT_RS19495 WP_008764133.1 16271 - 17414 (+) NC_004663.1:5029186-5030329 -
- BT_RS19500 WP_011108998.1 17488 - 18637 (+) NC_004663.1:5030403-5031552 -
- BT_RS19505 WP_008764135.1 18717 - 21093 (+) NC_004663.1:5031632-5034008 -
trpS BT_RS19510 WP_008760790.1 21174 - 22275 (-) NC_004663.1:5034089-5035190 6.1.1.2
- BT_RS19515 WP_072067671.1 22511 - 22537 (+) NC_004663.1:5035426-5035452 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2574 (+) CDS No
- 3043 - 6174 (+) TC: gnl|TC-DB|Q93TH9|1.B.14.6.2 Yes
- 6186 - 8117 (+) other Yes
- 8146 - 8841 (+) other Yes
- 8860 - 10767 (+) other Yes
- 10799 - 13066 (+) CAZyme: GH92 Yes
- 13105 - 15081 (+) other Yes
- 15115 - 16245 (+) other Yes
- 16272 - 17414 (+) other Yes
- 17489 - 18637 (+) CAZyme: GH99 Yes
- 18718 - 21093 (+) TC: gnl|TC-DB|Q8F8J8|9.B.14.1.10 Yes
trpS 21175 - 22275 (-) CDS No
- 22512 - 22537 (+) CDS No

PUL ID

PUL0162

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.