Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0173

PubMed

27177202, Carbohydr Res. 2016 Jul 22;430:24-28. doi: 10.1016/j.carres.2016.04.025. Epub 2016 Apr 29.

Characterization method

sequence homology analysis,NMR,sugar utilization assay

Genomic accession number

AB812065

Nucelotide position range

1-21325

Substrate

O-antigen

Loci

None

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ01786.1 0 - 724 (+) AB812065.1:1-725 -
galF - BAQ01787.1 886 - 1792 (+) AB812065.1:887-1793 -
- - BAQ01788.1 2178 - 2661 (+) AB812065.1:2179-2662 -
- - BAQ01789.1 2696 - 3692 (+) AB812065.1:2697-3693 -
wzx - BAQ01790.1 3955 - 5095 (+) AB812065.1:3956-5096 -
- - BAQ01791.1 5108 - 6071 (+) AB812065.1:5109-6072 -
- - BAQ01792.1 6121 - 7363 (+) AB812065.1:6122-7364 -
wzy - BAQ01793.1 7372 - 8578 (+) AB812065.1:7373-8579 -
- - BAQ01794.1 8549 - 9257 (+) AB812065.1:8550-9258 -
- - BAQ01795.1 9253 - 10333 (+) AB812065.1:9254-10334 -
gmd - BAQ01796.1 10333 - 11452 (+) AB812065.1:10334-11453 -
fcl - BAQ01797.1 11455 - 12421 (+) AB812065.1:11456-12422 -
gmm - BAQ01798.1 12423 - 12885 (+) AB812065.1:12424-12886 -
manC - BAQ01799.1 12890 - 14297 (+) AB812065.1:12891-14298 -
- - BAQ01800.1 14296 - 15043 (+) AB812065.1:14297-15044 -
manB - BAQ01801.1 15048 - 16473 (+) AB812065.1:15049-16474 -
gnd - BAQ01802.1 16669 - 18076 (+) AB812065.1:16670-18077 -
ugd - BAQ01803.1 18323 - 19490 (+) AB812065.1:18324-19491 -
wzz - BAQ01804.1 19579 - 20617 (+) AB812065.1:19580-20618 -
hisI - BAQ01805.1 20713 - 21325 (-) AB812065.1:20714-21326 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 724 (+) CDS No
- 887 - 1792 (+) CDS No
- 2179 - 2661 (+) CDS No
- 2697 - 3692 (+) CDS No
- 3956 - 5095 (+) CAZyme: GT4 No
- 5109 - 6071 (+) CDS No
- 6122 - 7363 (+) CDS No
- 7373 - 8578 (+) CAZyme: GT4 No
- 8550 - 9257 (+) CDS No
- 9254 - 10333 (+) CDS No
- 10334 - 11452 (+) CDS No
- 11456 - 12421 (+) CDS No
- 12424 - 12885 (+) STP: STP|NUDIX No
- 12891 - 14297 (+) CAZyme: GT2|GT2_Glycos_transf_2 No
- 14297 - 15043 (+) CDS No
- 15049 - 16473 (+) CDS No
- 16670 - 18076 (+) CDS No
- 18324 - 19490 (+) CDS No
- 19580 - 20617 (+) CDS No
- 20714 - 21325 (-) CDS No

PUL ID

PUL0173

PubMed

27177202, Carbohydr Res. 2016 Jul 22;430:24-28. doi: 10.1016/j.carres.2016.04.025. Epub 2016 Apr 29.

Title

Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal.

Author

Duan Z, Senchenkova SN, Guo X, Perepelov AV, Shashkov AS, Liu B, Knirel YA

Abstract

The lipopolysaccharide of Escherichia coli O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coli O156 was analyzed and shown to be consistent with the O-polysaccharide structure.