Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0177

PubMed

27293097, Biochemistry (Mosc). 2016 Apr;81(4):401-6. doi: 10.1134/S0006297916040106.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB811600

Nucelotide position range

1-16476

Substrate

O-antigen

Loci

BAQ00630.1-BAQ00644.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ00630.1 0 - 903 (+) AB811600.1:1-904 -
galF - BAQ00631.1 1065 - 1971 (+) AB811600.1:1066-1972 -
- - BAQ00632.1 2320 - 3280 (+) AB811600.1:2321-3281 -
wzx - BAQ00633.1 3352 - 4612 (+) AB811600.1:3353-4613 -
wzy - BAQ00634.1 4624 - 5728 (+) AB811600.1:4625-5729 -
- - BAQ00635.1 5728 - 6826 (+) AB811600.1:5729-6827 -
fnlA - BAQ00636.1 6832 - 7867 (+) AB811600.1:6833-7868 -
fnlB - BAQ00637.1 7868 - 8975 (+) AB811600.1:7869-8976 -
fnlC - BAQ00638.1 8971 - 10102 (+) AB811600.1:8972-10103 -
- - BAQ00639.1 10101 - 11310 (+) AB811600.1:10102-11311 -
- - BAQ00640.1 11300 - 11708 (+) AB811600.1:11301-11709 -
gnd - BAQ00641.1 11820 - 13227 (+) AB811600.1:11821-13228 -
ugd - BAQ00642.1 13475 - 14642 (+) AB811600.1:13476-14643 -
wzz - BAQ00643.1 14731 - 15769 (+) AB811600.1:14732-15770 -
hisI - BAQ00644.1 15864 - 16476 (-) AB811600.1:15865-16477 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 903 (+) CDS No
- 1066 - 1971 (+) TC: gnl|TC-DB|P75905|4.D.1.1.3 Yes
- 2321 - 3280 (+) other Yes
- 3353 - 4612 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 4625 - 5728 (+) CAZyme: GT4 Yes
- 5729 - 6826 (+) other Yes
- 6833 - 7867 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 7869 - 8975 (+) CDS No
- 8972 - 10102 (+) CDS No
- 10102 - 11310 (+) CDS No
- 11301 - 11708 (+) CDS No
- 11821 - 13227 (+) CDS No
- 13476 - 14642 (+) CDS No
- 14732 - 15769 (+) CDS No
- 15865 - 16476 (-) CDS No

PUL ID

PUL0177

PubMed

27293097, Biochemistry (Mosc). 2016 Apr;81(4):401-6. doi: 10.1134/S0006297916040106.

Title

Structure and Biosynthesis Gene Cluster of the O-Antigen of Escherichia coli O12.

Author

Senchenkova SN, Zhang Y, Perepelov AV, Guo X, Shashkov AS, Liu B, Knirel YA

Abstract

Two polysaccharides were isolated from Escherichia coli O12, the major being identified as the O12-antigen and the minor as the K5-antigen. The polysaccharides were studied by sugar analysis, Smith degradation, and one- and two-dimensional (1)H and (13)C NMR spectroscopy. As a result, the following structure of the O12-polysaccharide was elucidated, which, to our knowledge, has not been hitherto found in bacterial carbohydrates: -->2)-beta-d-Glcp-(1-->6)-alpha-d-GlcpNAc-(1-->3)-alpha-l-FucpNAc-(1-->3)-beta-d-GlcpNAc-(1-->. The -->4)-beta-d-GlcpA-(1-->4)-alpha-d-GlcpNAc-(1--> structure established for the K5-polysaccharide (heparosan) is previously known. Functions of genes in the O-antigen biosynthesis gene cluster of E. coli O12 were assigned by comparison with sequences in the available databases and found to be consistent with the O12-polysaccharide structure.