dbCAN-PUL

PUL ID	PUL0198
PubMed	26706815, Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23.
Characterization method	NMR,sugar utilization assay
Genomic accession number	AB812043
Nucelotide position range	1-14195
Substrate	O-antigen
Loci	BAQ01414.1-BAQ01426.1
Species	Escherichia coli/562
Degradation or Biosynthesis	biosynthesis

PUL ID	PUL0198
PubMed	26706815, Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23.
Title	Structure and gene cluster of the o-antigen of Escherichia coli o96.
Author	Guo X, Senchenkova SN, Shashkov AS, Perepelov AV, Liu B, Knirel YA
Abstract	Mild acid degradation of the lipopolysaccharide of Escherichia coli O96 afforded a mixture of two polysaccharides. The following structure of the pentasaccharide repeating unit of the major polymer was established by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy: [Formula: see text]. The O-antigen gene cluster of E. coli O96 between conserved galF and gnd genes was found to be consistent with this structure, and hence, the major polysaccharide represents the O96-antigen. The O96-antigen structure and gene cluster are similar to those of E. coli O170, and two proteins encoded in the gene clusters of both bacteria were putatively assigned a function of galactofuranosyltransferases. The minor polymer has the same structure as a peptidoglycan-related polysaccharide reported earlier in Providencia alcalifeciens O45 and several other O-serogoups of this species (Ovchinnikova OG, Liu B, Kocharova NA, Shashkov AS, Kondakova AN, Siwinska M, Feng L, Rozalski A, Wang L, Knirel YA. Biochemistry (Moscow) 2012;77:609-15) --> 4)-beta-D-GlcpNAc-(1 --> 4)-beta-D-GlcpNAc3(Rlac-lAla)-(1 --> where Rlac-lAla indicates (R)-1-[(S)-1-carboxyethylaminocarbonyl]ethyl.