PUL ID

PUL0198

PubMed

26706815, Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23.

Characterization method

NMR, sugar utilization assay

Genomic accession number

AB812043

Nucelotide position range

1-14195

Substrate

O-antigen

Loci

BAQ01414.1-BAQ01426.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ01414.1 0 - 906 (+) AB812043.1:1-907 -
galF - BAQ01415.1 1068 - 1974 (+) AB812043.1:1069-1975 -
wzx - BAQ01416.1 2308 - 3556 (+) AB812043.1:2309-3557 -
glf - BAQ01417.1 3567 - 4665 (+) AB812043.1:3568-4666 -
- - BAQ01418.1 4661 - 5576 (+) AB812043.1:4662-5577 -
wzy - BAQ01419.1 5613 - 6864 (+) AB812043.1:5614-6865 -
- - BAQ01420.1 6868 - 7990 (+) AB812043.1:6869-7991 -
- - BAQ01421.1 7967 - 8930 (+) AB812043.1:7968-8931 -
- - BAQ01422.1 8944 - 9868 (+) AB812043.1:8945-9869 -
gnd - BAQ01423.1 9986 - 11393 (+) AB812043.1:9987-11394 -
ugd - BAQ01424.1 11641 - 12808 (+) AB812043.1:11642-12809 -
wzz - BAQ01425.1 12897 - 13932 (+) AB812043.1:12898-13933 -
hisI - BAQ01426.1 14028 - 14195 (-) AB812043.1:14029-14196 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 906 (+) CDS No
- 1069 - 1974 (+) CDS No
- 2309 - 3556 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 3568 - 4665 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 4662 - 5576 (+) other Yes
- 5614 - 6864 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 6869 - 7990 (+) CDS No
- 7968 - 8930 (+) CDS No
- 8945 - 9868 (+) CDS No
- 9987 - 11393 (+) CDS No
- 11642 - 12808 (+) CDS No
- 12898 - 13932 (+) CDS No
- 14029 - 14195 (-) CDS No

PUL ID

PUL0198

PubMed

26706815, Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23.

Title

Structure and gene cluster of the o-antigen of Escherichia coli o96.

Author

Guo X, Senchenkova SN, Shashkov AS, Perepelov AV, Liu B, Knirel YA

Abstract

Mild acid degradation of the lipopolysaccharide of Escherichia coli O96 afforded a mixture of two polysaccharides. The following structure of the pentasaccharide repeating unit of the major polymer was established by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy: [Formula: see text]. The O-antigen gene cluster of E. coli O96 between conserved galF and gnd genes was found to be consistent with this structure, and hence, the major polysaccharide represents the O96-antigen. The O96-antigen structure and gene cluster are similar to those of E. coli O170, and two proteins encoded in the gene clusters of both bacteria were putatively assigned a function of galactofuranosyltransferases. The minor polymer has the same structure as a peptidoglycan-related polysaccharide reported earlier in Providencia alcalifeciens O45 and several other O-serogoups of this species (Ovchinnikova OG, Liu B, Kocharova NA, Shashkov AS, Kondakova AN, Siwinska M, Feng L, Rozalski A, Wang L, Knirel YA. Biochemistry (Moscow) 2012;77:609-15) --> 4)-beta-D-GlcpNAc-(1 --> 4)-beta-D-GlcpNAc3(Rlac-lAla)-(1 --> where Rlac-lAla indicates (R)-1-[(S)-1-carboxyethylaminocarbonyl]ethyl.