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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0200

PubMed

26451883, Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB812066

Nucelotide position range

1-14841

Substrate

O-antigen

Loci

BAQ01806.1-BAQ01818.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ01806.1 0 - 867 (+) AB812066.1:1-868 -
gne - BAQ01807.1 1024 - 2020 (+) AB812066.1:1025-2021 -
galF - BAQ01808.1 2262 - 3156 (+) AB812066.1:2263-3157 -
wzx - BAQ01809.1 3505 - 4753 (+) AB812066.1:3506-4754 -
- - BAQ01810.1 4742 - 5744 (+) AB812066.1:4743-5745 -
- - BAQ01811.1 5743 - 6676 (+) AB812066.1:5744-6677 -
wzy - BAQ01812.1 6672 - 7773 (+) AB812066.1:6673-7774 -
- - BAQ01813.1 7769 - 8789 (+) AB812066.1:7770-8790 -
- - BAQ01814.1 8795 - 9614 (+) AB812066.1:8796-9615 -
gnd - BAQ01815.1 9762 - 11169 (+) AB812066.1:9763-11170 -
ugd - BAQ01816.1 12127 - 13294 (+) AB812066.1:12128-13295 -
wzz - BAQ01817.1 13383 - 14418 (+) AB812066.1:13384-14419 -
hisI - BAQ01818.1 14514 - 14841 (-) AB812066.1:14515-14842 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 867 (+) CDS No
- 1025 - 2020 (+) CDS No
- 2263 - 3156 (+) CDS No
- 3506 - 4753 (+) CAZyme: GT2 No
- 4743 - 5744 (+) CDS No
- 5744 - 6676 (+) CDS No
- 6673 - 7773 (+) CAZyme: GT2 No
- 7770 - 8789 (+) CDS No
- 8796 - 9614 (+) CDS No
- 9763 - 11169 (+) CDS No
- 12128 - 13294 (+) CDS No
- 13384 - 14418 (+) CDS No
- 14515 - 14841 (-) CDS No

PUL ID

PUL0200

PubMed

26451883, Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16.

Title

Structure and genetics of biosynthesis of the glycosyl phosphate-containing O-polysaccharide of Escherichia coli O160.

Author

Perepelov AV, Guo X, Senchenkova SN, Shashkov AS, Knirel YA

Abstract

On mild acid degradation of the lipopolysaccharide of Escherichia coli O160, the O-polysaccharide was cleaved by acid-labile glycosyl phosphate linkages in the main chain. The resultant oligosaccharide and the alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopies, and the following structure of the branched pentasaccharide repeating unit of the O-polysaccharide was established: The O-antigen gene cluster of E. coli O160 was found to be consistent with the O-polysaccharide structure established.