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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0201

PubMed

26382081, Carbohydr Res. 2015 Nov 19;417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB812070.1

Nucelotide position range

1-13820

Substrate

O-antigen

Loci

BAQ01872.1-BAQ01884.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ01872.1 0 - 690 (+) AB812070.1:1-691 -
galF - BAQ01873.1 852 - 1758 (+) AB812070.1:853-1759 -
wzx - BAQ01874.1 2091 - 3339 (+) AB812070.1:2092-3340 -
glf - BAQ01875.1 3350 - 4457 (+) AB812070.1:3351-4458 -
- - BAQ01876.1 4453 - 5386 (+) AB812070.1:4454-5387 -
- - BAQ01877.1 5369 - 6176 (+) AB812070.1:5370-6177 -
wzy - BAQ01878.1 6175 - 7348 (+) AB812070.1:6176-7349 -
- - BAQ01879.1 7331 - 8186 (+) AB812070.1:7332-8187 -
- - BAQ01880.1 8187 - 9105 (+) AB812070.1:8188-9106 -
gnd - BAQ01881.1 9166 - 10573 (+) AB812070.1:9167-10574 -
ugd - BAQ01882.1 10821 - 11988 (+) AB812070.1:10822-11989 -
wzz - BAQ01883.1 12077 - 13112 (+) AB812070.1:12078-13113 -
hisI - BAQ01884.1 13208 - 13820 (-) AB812070.1:13209-13821 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 690 (+) CDS No
- 853 - 1758 (+) CDS No
- 2092 - 3339 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes
- 3351 - 4457 (+) other Yes
- 4454 - 5386 (+) other Yes
- 5370 - 6176 (+) other Yes
- 6176 - 7348 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 7332 - 8186 (+) CDS No
- 8188 - 9105 (+) CDS No
- 9167 - 10573 (+) CDS No
- 10822 - 11988 (+) CDS No
- 12078 - 13112 (+) CDS No
- 13209 - 13820 (-) CDS No

PUL ID

PUL0201

PubMed

26382081, Carbohydr Res. 2015 Nov 19;417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28.

Title

Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170.

Author

Shashkov AS, Wang T, Perepelov AV, Weintraub A, Liu B, Widmalm G, Knirel YA

Abstract

Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E.coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E.coli O170, the branched O-polysaccharide chain was partially cleaved at beta-d-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D (1)H and (13)C NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure.