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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0202

PubMed

26342864, Carbohydr Res. 2015 Oct 30;416:32-6. doi: 10.1016/j.carres.2015.08.008. Epub 2015 Aug 18.

Characterization method

NMR,sugar utilization assay

Genomic accession number

AB811619

Nucelotide position range

1-19971

Substrate

O-antigen

Loci

BAQ00920.1-BAQ00938.1

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ00920.1 0 - 906 (+) AB811619.1:1-907 -
gne - BAQ00921.1 1063 - 2059 (+) AB811619.1:1064-2060 -
galF - BAQ00922.1 2301 - 3195 (+) AB811619.1:2302-3196 -
wzx - BAQ00923.1 3512 - 4826 (+) AB811619.1:3513-4827 -
- - BAQ00924.1 4838 - 5585 (+) AB811619.1:4839-5586 -
- - BAQ00925.1 5581 - 6103 (+) AB811619.1:5582-6104 -
- - BAQ00926.1 6081 - 7170 (+) AB811619.1:6082-7171 -
wzy - BAQ00927.1 7156 - 8368 (+) AB811619.1:7157-8369 -
- - BAQ00928.1 8364 - 9414 (+) AB811619.1:8365-9415 -
gmd - BAQ00929.1 9410 - 10529 (+) AB811619.1:9411-10530 -
fcl - BAQ00930.1 10532 - 11498 (+) AB811619.1:10533-11499 -
gmm - BAQ00931.1 11500 - 11956 (+) AB811619.1:11501-11957 -
manC - BAQ00932.1 11952 - 13371 (+) AB811619.1:11953-13372 -
- - BAQ00933.1 13424 - 14183 (+) AB811619.1:13425-14184 -
manB - BAQ00934.1 14207 - 15578 (+) AB811619.1:14208-15579 -
gnd - BAQ00935.1 15741 - 17148 (+) AB811619.1:15742-17149 -
ugd - BAQ00936.1 17395 - 18562 (+) AB811619.1:17396-18563 -
wzz - BAQ00937.1 18651 - 19689 (+) AB811619.1:18652-19690 -
hisI - BAQ00938.1 19785 - 19971 (-) AB811619.1:19786-19972 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 906 (+) CDS No
- 1064 - 2059 (+) CDS No
- 2302 - 3195 (+) CDS No
- 3513 - 4826 (+) CDS No
- 4839 - 5585 (+) CDS No
- 5582 - 6103 (+) CDS No
- 6082 - 7170 (+) CDS No
- 7157 - 8368 (+) CAZyme: GT4 No
- 8365 - 9414 (+) CDS No
- 9411 - 10529 (+) CDS No
- 10533 - 11498 (+) CDS No
- 11501 - 11956 (+) STP: STP|NUDIX No
- 11953 - 13371 (+) CAZyme: GT2|GT2_Glycos_transf_2 No
- 13425 - 14183 (+) CDS No
- 14208 - 15578 (+) CDS No
- 15742 - 17148 (+) CDS No
- 17396 - 18562 (+) CDS No
- 18652 - 19689 (+) CDS No
- 19786 - 19971 (-) CDS No

PUL ID

PUL0202

PubMed

26342864, Carbohydr Res. 2015 Oct 30;416:32-6. doi: 10.1016/j.carres.2015.08.008. Epub 2015 Aug 18.

Title

Structure and gene cluster of the O-antigen of Escherichia coli O43.

Author

Perepelov AV, Guo X, Filatov AV, Liu B, Knirel YA

Abstract

The O-polysaccharide (O-antigen) of Escherichia coli O43 was isolated from the lipopolysaccharide and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: [Formula: see text] Functions of genes in the O-antigen gene cluster of E. coli O43 were assigned by a comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.