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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0212

PubMed

25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.

Characterization method

qRT-PCR

Genomic accession number

NC_008618.1

Nucelotide position range

2006567-2020309

Substrate

galactooligosaccharide

Loci

BAD_RS08320- BAD_RS08355

Species

Bifidobacterium adolescentis/1680

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BAD_RS08320 WP_011743876.1 0 - 3075 (-) NC_008618.1:2006567-2009642 -
- BAD_RS08325 WP_011743877.1 3430 - 6580 (+) NC_008618.1:2009997-2013147 -
- BAD_RS08330 WP_034983933.1 6773 - 7616 (+) NC_008618.1:2013340-2014183 -
- BAD_RS08335 WP_011743879.1 7776 - 8808 (+) NC_008618.1:2014343-2015375 -
- BAD_RS08340 WP_011743880.1 9062 - 10370 (+) NC_008618.1:2015629-2016937 -
- BAD_RS08345 WP_003806528.1 10550 - 11486 (+) NC_008618.1:2017117-2018053 -
- BAD_RS08350 WP_003806530.1 11547 - 12426 (+) NC_008618.1:2018114-2018993 -
- BAD_RS08355 WP_011743881.1 12612 - 13743 (+) NC_008618.1:2019179-2020310 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3075 (-) TC: gnl|TC-DB|A9BJX0|3.A.3.2.21 Yes
- 3431 - 6580 (+) CAZyme: GH2 Yes
- 6774 - 7616 (+) TC: gnl|TC-DB|Q8G3M7|9.B.36.1.3 Yes
- 7777 - 8808 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
- 9063 - 10370 (+) STP: STP|SBP_bac_1 Yes
- 10551 - 11486 (+) TC: gnl|TC-DB|Q8RJU9|3.A.1.1.18 Yes
- 11548 - 12426 (+) TC: gnl|TC-DB|A5LBQ4|3.A.1.1.47 Yes
- 12613 - 13743 (+) CDS No

PUL ID

PUL0212

PubMed

25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.

Title

Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon.

Author

Akiyama T, Kimura K, Hatano H

Abstract

Galactooligosaccharides (GOS) possess prebiotic properties that specifically increase the number of bifidobacteria in the human intestine, thus giving health benefits to the host. Although the bifidogenic effect of GOS has been demonstrated in numerous studies, the utilization of GOS by specific bifidobacteria remains unclear. The goal of our study was to elucidate GOS consumption by specific bifidobacteria and gain insights into the mechanism. First, we examined GOS consumption by 14 bifidobacterial strains belonging to seven different species by comparing growth rate, carbohydrate consumption, and acid production. We then performed a transcription analysis in the case of one strong GOS consumer, Bifidobacterium adolescentis YIT 4011(T), to predict the operon contributing to GOS use. The study indicated the contribution of an operon consisted of LacS symporter and beta-galactosidase to bifidobacterial GOS consumption.