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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0214

PubMed

25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.

Characterization method

qRT-PCR

Genomic accession number

NC_008618.1

Nucelotide position range

1763016-1778475

Substrate

galactooligosaccharide

Loci

BAD_RS07390-BAD_RS07430

Species

Bifidobacterium adolescentis/1680

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BAD_RS07390 WP_011743697.1 0 - 1074 (-) NC_008618.1:1763016-1764090 -
- BAD_RS07395 WP_041777416.1 1118 - 3221 (-) NC_008618.1:1764134-1766237 -
- BAD_RS07400 WP_011743699.1 3276 - 5532 (-) NC_008618.1:1766292-1768548 -
- BAD_RS07405 WP_003809794.1 5573 - 6407 (-) NC_008618.1:1768589-1769423 -
- BAD_RS07410 WP_003809796.1 6403 - 7276 (-) NC_008618.1:1769419-1770292 -
- BAD_RS07415 WP_050731469.1 7467 - 8742 (-) NC_008618.1:1770483-1771758 -
pdxT BAD_RS07420 WP_041777551.1 9295 - 9988 (-) NC_008618.1:1772311-1773004 -
pdxS BAD_RS07425 WP_011743702.1 10075 - 10951 (-) NC_008618.1:1773091-1773967 -
- BAD_RS07430 WP_113736347.1 11374 - 15460 (-) NC_008618.1:1774390-1778476 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1074 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
- 1119 - 3221 (-) CAZyme: GH42 Yes
- 3277 - 5532 (-) CAZyme: GH35 Yes
- 5574 - 6407 (-) TC: gnl|TC-DB|Q9WYR1|3.A.1.1.39 Yes
- 6404 - 7276 (-) TC: gnl|TC-DB|Q7WWQ9|3.A.1.1.20 Yes
- 7468 - 8742 (-) STP: STP|SBP_bac_1 Yes
pdxT 9296 - 9988 (-) other Yes
pdxS 10076 - 10951 (-) other Yes
- 11375 - 15460 (-) TC: gnl|TC-DB|Q3T7C8|3.A.1.201.15 Yes

PUL ID

PUL0214

PubMed

25483279, Biosci Biotechnol Biochem. 2015;79(4):664-72. doi: 10.1080/09168451.2014.987204. Epub 2014 Dec 6.

Title

Diverse galactooligosaccharides consumption by bifidobacteria: implications of beta-galactosidase--LacS operon.

Author

Akiyama T, Kimura K, Hatano H

Abstract

Galactooligosaccharides (GOS) possess prebiotic properties that specifically increase the number of bifidobacteria in the human intestine, thus giving health benefits to the host. Although the bifidogenic effect of GOS has been demonstrated in numerous studies, the utilization of GOS by specific bifidobacteria remains unclear. The goal of our study was to elucidate GOS consumption by specific bifidobacteria and gain insights into the mechanism. First, we examined GOS consumption by 14 bifidobacterial strains belonging to seven different species by comparing growth rate, carbohydrate consumption, and acid production. We then performed a transcription analysis in the case of one strong GOS consumer, Bifidobacterium adolescentis YIT 4011(T), to predict the operon contributing to GOS use. The study indicated the contribution of an operon consisted of LacS symporter and beta-galactosidase to bifidobacterial GOS consumption.