PUL ID

PUL0215

PubMed

25171165, Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17.

Characterization method

qPCR, enzyme activity assay

Genomic accession number

NC_010995.1

Nucelotide position range

3175359-3185628

Substrate

xyloglucan

Loci

CJA_RS13075-CJA_RS13090

Species

Cellvibrio japonicus/155077

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- CJA_RS13075 WP_012488300.1 0 - 2967 (-) NC_010995.1:3175359-3178326 -
- CJA_RS13080 WP_012488301.1 3007 - 4735 (-) NC_010995.1:3178366-3180094 -
- CJA_RS13085 WP_012488303.1 4933 - 7834 (-) NC_010995.1:3180292-3183193 -
- CJA_RS13090 WP_012488304.1 7921 - 10270 (-) NC_010995.1:3183280-3185629 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2967 (-) CAZyme: GH31 Yes
- 3008 - 4735 (-) CAZyme: GH35 Yes
- 4934 - 7834 (-) TC: gnl|TC-DB|Q9AAZ6|1.B.14.12.2 Yes
- 7922 - 10270 (-) CAZyme: GH95 Yes

PUL ID

PUL0215

PubMed

25171165, Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17.

Title

A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus.

Author

Larsbrink J, Thompson AJ, Lundqvist M, Gardner JG, Davies GJ, Brumer H

Abstract

The degradation of plant biomass by saprophytes is an ecologically important part of the global carbon cycle, which has also inspired a vast diversity of industrial enzyme applications. The xyloglucans (XyGs) constitute a family of ubiquitous and abundant plant cell wall polysaccharides, yet the enzymology of XyG saccharification is poorly studied. Here, we present the identification and molecular characterization of a complex genetic locus that is required for xyloglucan utilization by the model saprophyte Cellvibrio japonicus. In harness, transcriptomics, reverse genetics, enzyme kinetics, and structural biology indicate that the encoded cohort of an alpha-xylosidase, a beta-galactosidase, and an alpha-l-fucosidase is specifically adapted for efficient, concerted saccharification of dicot (fucogalacto)xyloglucan oligosaccharides following import into the periplasm via an associated TonB-dependent receptor. The data support a biological model of xyloglucan degradation by C. japonicus with striking similarities - and notable differences - to the complex polysaccharide utilization loci of the Bacteroidetes.