dbCAN-PUL

PUL ID	PUL0215
PubMed	25171165, Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17.
Characterization method	qPCR,enzyme activity assay
Genomic accession number	NC_010995.1
Nucelotide position range	3175359-3185628
Substrate	xyloglucan
Loci	CJA_RS13075-CJA_RS13090
Species	Cellvibrio japonicus/155077
Degradation or Biosynthesis	degradation

PUL ID	PUL0215
PubMed	25171165, Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17.
Title	A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus.
Author	Larsbrink J, Thompson AJ, Lundqvist M, Gardner JG, Davies GJ, Brumer H
Abstract	The degradation of plant biomass by saprophytes is an ecologically important part of the global carbon cycle, which has also inspired a vast diversity of industrial enzyme applications. The xyloglucans (XyGs) constitute a family of ubiquitous and abundant plant cell wall polysaccharides, yet the enzymology of XyG saccharification is poorly studied. Here, we present the identification and molecular characterization of a complex genetic locus that is required for xyloglucan utilization by the model saprophyte Cellvibrio japonicus. In harness, transcriptomics, reverse genetics, enzyme kinetics, and structural biology indicate that the encoded cohort of an alpha-xylosidase, a beta-galactosidase, and an alpha-l-fucosidase is specifically adapted for efficient, concerted saccharification of dicot (fucogalacto)xyloglucan oligosaccharides following import into the periplasm via an associated TonB-dependent receptor. The data support a biological model of xyloglucan degradation by C. japonicus with striking similarities - and notable differences - to the complex polysaccharide utilization loci of the Bacteroidetes.