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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0248

PubMed

24102883, Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10.

Characterization method

sequence homology analysis

Genomic accession number

AE016795.3

Nucelotide position range

775591-796807

Substrate

capsule polysaccharide

Loci

VV1_0774-VV1_0786

Species

Vibrio vulnificus/672

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- VV1_0774 AAO09278.1 0 - 1167 (-) AE016795.3:775591-776758 1.1.1.22
- VV1_0775 AAO09279.1 1342 - 3295 (-) AE016795.3:776933-778886 -
- VV1_0776 AAO09280.2 3319 - 3874 (-) AE016795.3:778910-779465 2.4.1.-
- VV1_0777 AAO09281.1 3877 - 4789 (-) AE016795.3:779468-780380 5.1.3.2
- VV1_0778 AAO09282.2 4785 - 5916 (-) AE016795.3:780376-781507 -
- VV1_3212 ADV91927.1 5902 - 7558 (-) AE016795.3:781493-783149 -
- VV1_3213 ADV91928.1 7520 - 8642 (-) AE016795.3:783111-784233 -
- VV1_3214 ADV91929.1 8638 - 10360 (-) AE016795.3:784229-785951 -
- VV1_3215 ADV91930.1 10328 - 11453 (-) AE016795.3:785919-787044 -
- VV1_3216 ADV91931.1 11406 - 12672 (-) AE016795.3:786997-788263 -
- VV1_3217 ADV91932.1 12671 - 13820 (-) AE016795.3:788262-789411 -
- VV1_0779 AAO09283.1 13859 - 15131 (-) AE016795.3:789450-790722 -
- VV1_0780 AAO09284.2 15319 - 16459 (-) AE016795.3:790910-792050 5.1.3.14
- VV1_0781 AAO09285.1 16527 - 18711 (-) AE016795.3:792118-794302 2.7.10.2
- VV1_0782 AAO09286.1 18707 - 18881 (-) AE016795.3:794298-794472 -
- VV1_0783 AAO09287.1 18921 - 19362 (-) AE016795.3:794512-794953 3.1.3.48
- VV1_0784 AAO09288.1 19379 - 19622 (-) AE016795.3:794970-795213 -
- VV1_0785 AAO09289.1 19639 - 19777 (-) AE016795.3:795230-795368 -
- VV1_0786 AAO09290.1 20080 - 21217 (-) AE016795.3:795671-796808 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1167 (-) CDS No
- 1343 - 3295 (-) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 3320 - 3874 (-) TC: gnl|TC-DB|917585193|9.B.18.1.3 Yes
- 3878 - 4789 (-) other Yes
- 4786 - 5916 (-) other Yes
- 5903 - 7558 (-) CAZyme: PL12_3|PL0 Yes
- 7521 - 8642 (-) other Yes
- 8639 - 10360 (-) other Yes
- 10329 - 11453 (-) other Yes
- 11407 - 12672 (-) other Yes
- 12672 - 13820 (-) other Yes
- 13860 - 15131 (-) other Yes
- 15320 - 16459 (-) other Yes
- 16528 - 18711 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 18708 - 18881 (-) other Yes
- 18922 - 19362 (-) other Yes
- 19380 - 19622 (-) other Yes
- 19640 - 19777 (-) other Yes
- 20081 - 21217 (-) TC: gnl|TC-DB|P0A932|1.B.18.3.8 Yes

PUL ID

PUL0248

PubMed

24102883, Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10.

Title

Role of capsular polysaccharide (CPS) in biofilm formation and regulation of CPS production by quorum-sensing in Vibrio vulnificus.

Author

Lee KJ, Kim JA, Hwang W, Park SJ, Lee KH

Abstract

Extracellular polysaccharides, such as lipopolysaccharide and loosely associated exopolysaccharides, are essential for Vibrio vulnificus to form biofilms. The role of another major component of the V. vulnificus extracellular matrix, capsular polysaccharide (CPS), which contributes to colony opacity, has been characterized in biofilm formation. A CPS-deficient mutant, whose wbpP gene encoding UDP-GlcNAc C4-epimerase was knocked out, formed significantly more biofilm than wild type, due to increased hydrophobicity of the cell surface, adherence to abiotic surfaces and cell aggregation. To elucidate the direct effect of CPS on biofilm structure, extracted CPS and a CPS-degrading enzyme, alpha-N-acetylgalactosaminidase, were added in biofilm assays, resulting in reduction and increment of biofilm sizes respectively. Therefore, it is suggested that CPS play a critical role in determining biofilm size by restricting continual growth of mature biofilms. Since CPS is required after maturation, CPS biosynthesis should be controlled in a cell density-dependent manner, e.g. by quorum-sensing (QS) regulation. Analysing transcription of the CPS gene cluster revealed that it was activated by SmcR, a QS master regulator, via binding to the upstream region of the cluster. Therefore, CPS was produced when biofilm cell density reached high enough to turn on QS regulation and limited biofilms to appropriate sizes.