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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0250

PubMed

24102883, Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10.

Characterization method

sequence homology analysis

Genomic accession number

BA000037.2

Nucelotide position range

343944-377650

Substrate

capsule polysaccharide

Loci

VV_0337-VV0366

Species

Vibrio vulnificus/672

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

VV0337 - BAC93101.1 0 - 1233 (+) BA000037.2:343944-345177 -
VV0338 - BAC93102.1 1372 - 1744 (+) BA000037.2:345316-345688 -
VV0339 - BAC93103.1 1901 - 2342 (+) BA000037.2:345845-346286 -
VV0340 - BAC93104.1 2576 - 4757 (+) BA000037.2:346520-348701 -
VV0341 - BAC93105.1 4825 - 5965 (+) BA000037.2:348769-349909 -
VV0342 - BAC93106.1 6046 - 7309 (+) BA000037.2:349990-351253 -
VV0343 - BAC93107.1 7256 - 8576 (+) BA000037.2:351200-352520 -
VV0344 - BAC93108.1 8576 - 9794 (+) BA000037.2:352520-353738 -
VV0345 - BAC93109.1 9777 - 10494 (+) BA000037.2:353721-354438 -
VV0346 - BAC93110.1 10450 - 10870 (+) BA000037.2:354394-354814 -
VV0347 - BAC93111.1 11351 - 12401 (+) BA000037.2:355295-356345 -
VV0348 - BAC93112.1 12397 - 13480 (+) BA000037.2:356341-357424 -
VV0349 - BAC93113.1 13494 - 14616 (+) BA000037.2:357438-358560 -
VV0350 - BAC93114.1 14644 - 15652 (+) BA000037.2:358588-359596 -
VV0351 - BAC93115.1 15662 - 16145 (+) BA000037.2:359606-360089 -
VV0352 - BAC93116.1 16141 - 17551 (+) BA000037.2:360085-361495 -
VV0353 - BAC93117.1 17554 - 18964 (+) BA000037.2:361498-362908 -
VV0354 - BAC93118.1 18960 - 20112 (+) BA000037.2:362904-364056 -
VV0355 - BAC93119.1 20108 - 20729 (+) BA000037.2:364052-364673 -
VV0356 - BAC93120.1 20715 - 21498 (+) BA000037.2:364659-365442 -
VV0357 - BAC93121.1 21541 - 23683 (+) BA000037.2:365485-367627 -
VV0358 - BAC93122.1 23679 - 25491 (+) BA000037.2:367623-369435 -
VV0359 - BAC93123.1 25108 - 25231 (+) BA000037.2:369052-369175 -
VV0360 - BAC93124.1 25490 - 26750 (+) BA000037.2:369434-370694 -
VV0361 - BAC93125.1 26730 - 27348 (+) BA000037.2:370674-371292 -
VV0362 - BAC93126.1 27283 - 28012 (+) BA000037.2:371227-371956 -
VV0363 - BAC93127.1 28165 - 29341 (+) BA000037.2:372109-373285 -
VV0364 - BAC93128.1 29456 - 31409 (+) BA000037.2:373400-375353 -
VV0365 - BAC93129.1 31569 - 32751 (+) BA000037.2:375513-376695 -
VV0366 - BAC93130.1 32876 - 33707 (+) BA000037.2:376820-377651 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1233 (+) TC: gnl|TC-DB|P0A932|1.B.18.3.8 Yes
- 1373 - 1744 (+) other Yes
- 1902 - 2342 (+) other Yes
- 2577 - 4757 (+) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 4826 - 5965 (+) other Yes
- 6047 - 7309 (+) other Yes
- 7257 - 8576 (+) other Yes
- 8577 - 9794 (+) other Yes
- 9778 - 10494 (+) other Yes
- 10451 - 10870 (+) other Yes
- 11352 - 12401 (+) CAZyme: GT4 Yes
- 12398 - 13480 (+) CAZyme: GT4 Yes
- 13495 - 14616 (+) other Yes
- 14645 - 15652 (+) other Yes
- 15663 - 16145 (+) STP: STP|NUDIX Yes
- 16142 - 17551 (+) other Yes
- 17555 - 18964 (+) other Yes
- 18961 - 20112 (+) other Yes
- 20109 - 20729 (+) other Yes
- 20716 - 21498 (+) other Yes
- 21542 - 23683 (+) other Yes
- 23680 - 25491 (+) CAZyme: PL12|PL0 Yes
- 25109 - 25231 (+) other Yes
- 25491 - 26750 (+) CAZyme: GT4 Yes
- 26731 - 27348 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 27284 - 28012 (+) other Yes
- 28166 - 29341 (+) other Yes
- 29457 - 31409 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 31570 - 32751 (+) CDS No
- 32877 - 33707 (+) CDS No

PUL ID

PUL0250

PubMed

24102883, Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10.

Title

Role of capsular polysaccharide (CPS) in biofilm formation and regulation of CPS production by quorum-sensing in Vibrio vulnificus.

Author

Lee KJ, Kim JA, Hwang W, Park SJ, Lee KH

Abstract

Extracellular polysaccharides, such as lipopolysaccharide and loosely associated exopolysaccharides, are essential for Vibrio vulnificus to form biofilms. The role of another major component of the V. vulnificus extracellular matrix, capsular polysaccharide (CPS), which contributes to colony opacity, has been characterized in biofilm formation. A CPS-deficient mutant, whose wbpP gene encoding UDP-GlcNAc C4-epimerase was knocked out, formed significantly more biofilm than wild type, due to increased hydrophobicity of the cell surface, adherence to abiotic surfaces and cell aggregation. To elucidate the direct effect of CPS on biofilm structure, extracted CPS and a CPS-degrading enzyme, alpha-N-acetylgalactosaminidase, were added in biofilm assays, resulting in reduction and increment of biofilm sizes respectively. Therefore, it is suggested that CPS play a critical role in determining biofilm size by restricting continual growth of mature biofilms. Since CPS is required after maturation, CPS biosynthesis should be controlled in a cell density-dependent manner, e.g. by quorum-sensing (QS) regulation. Analysing transcription of the CPS gene cluster revealed that it was activated by SmcR, a QS master regulator, via binding to the upstream region of the cluster. Therefore, CPS was produced when biofilm cell density reached high enough to turn on QS regulation and limited biofilms to appropriate sizes.