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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0252

PubMed

23996813, Biotechnol Bioeng. 2014 Jan;111(1):165-73. doi: 10.1002/bit.24994. Epub 2013 Jul 30.

Characterization method

gene chips

Genomic accession number

AE015928.1

Nucelotide position range

5419109-5433257

Substrate

mucin,O-glycan

Loci

BT_4132-BT_4137

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_4132 AAO79237.1 0 - 1179 (-) AE015928.1:5419109-5420288 -
- BT_4133 AAO79238.1 1194 - 2457 (-) AE015928.1:5420303-5421566 -
- BT_4134 AAO79239.1 2489 - 4412 (-) AE015928.1:5421598-5423521 -
- BT_4135 AAO79240.1 4422 - 7569 (-) AE015928.1:5423531-5426678 -
- BT_4136 AAO79241.1 7900 - 9718 (-) AE015928.1:5427009-5428827 -
- BT_4137 AAO79242.1 10216 - 14149 (+) AE015928.1:5429325-5433258 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1179 (-) CAZyme: CBM32 Yes
- 1195 - 2457 (-) other Yes
- 2490 - 4412 (-) other Yes
- 4423 - 7569 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 7901 - 9718 (-) CAZyme: GH29 Yes
- 10217 - 14149 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 No

PUL ID

PUL0252

PubMed

23996813, Biotechnol Bioeng. 2014 Jan;111(1):165-73. doi: 10.1002/bit.24994. Epub 2013 Jul 30.

Title

Regulated expression of polysaccharide utilization and capsular biosynthesis loci in biofilm and planktonic Bacteroides thetaiotaomicron during growth in chemostats.

Author

TerAvest MA, He Z, Rosenbaum MA, Martens EC, Cotta MA, Gordon JI, Angenent LT

Abstract

Bacteroides thetaiotaomicron is a prominent member of the human distal gut microbiota that specializes in breaking down diet and host-derived polysaccharides. While polysaccharide utilization has been well studied in B. thetaiotaomicron, other aspects of its behavior are less well characterized, including the factors that allow it to maintain itself in the gut. Biofilm formation may be a mechanism for bacterial retention in the gut. Therefore, we used custom GeneChips to compare the transcriptomes of biofilm and planktonic B. thetaiotaomicron during growth in mono-colonized chemostats. We identified 1,154 genes with a fold-change greater than 2, with confidence greater than or equal to 95%. Among the prominent changes observed in biofilm populations were: (i) greater expression of genes in polysaccharide utilization loci that are involved in foraging of O-glycans normally found in the gut mucosa; and (ii) regulated expression of capsular polysaccharide biosynthesis loci. Hierarchical clustering of the data with different datasets, which were obtained during growth under a range of conditions in minimal media and in intestinal tracts of gnotobiotic mice, revealed that within this group of differentially expressed genes, biofilm communities were more similar to the in vivo samples than to planktonic cells and exhibited features of substrate limitation. The current study also validates the use of chemostats as an in vitro "gnotobiotic" model to study gene expression of attached populations of this bacterium. This is important to gut microbiota research, because bacterial attachment and the consequences of disruptions in attachment are difficult to study in vivo.