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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0280

PubMed

23226442, PLoS One. 2012;7(11):e50987. doi: 10.1371/journal.pone.0050987. Epub 2012 Nov 30.

Characterization method

microarray,qRT-PCR

Genomic accession number

CP000407.1

Nucelotide position range

552507-570820

Substrate

capsule polysaccharide

Loci

SSU05_0564-SSU05_0578

Species

Streptococcus suis/1307

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SSU05_0564 ABP89530.1 0 - 1452 (+) CP000407.1:552507-553959 -
- SSU05_0565 ABP89531.1 1469 - 2159 (+) CP000407.1:553976-554666 -
- SSU05_0566 ABP89532.1 2168 - 2864 (+) CP000407.1:554675-555371 -
- SSU05_0567 ABP89533.1 2889 - 3618 (+) CP000407.1:555396-556125 -
- SSU05_0568 ABP89534.1 3642 - 4881 (+) CP000407.1:556149-557388 -
- SSU05_0569 ABP89535.1 5055 - 6225 (+) CP000407.1:557562-558732 -
- SSU05_0570 ABP89536.1 6228 - 7386 (+) CP000407.1:558735-559893 -
- SSU05_0571 ABP89537.1 7699 - 9145 (+) CP000407.1:560206-561652 -
- SSU05_0572 ABP89538.1 9179 - 10412 (+) CP000407.1:561686-562919 -
- SSU05_0573 ABP89539.1 10550 - 11549 (+) CP000407.1:563057-564056 -
- SSU05_0574 ABP89540.1 11541 - 12546 (+) CP000407.1:564048-565053 -
- SSU05_0575 ABP89541.1 14388 - 15204 (+) CP000407.1:566895-567711 -
- SSU05_0576 ABP89542.1 15243 - 15360 (+) CP000407.1:567750-567867 -
- SSU05_0577 ABP89543.1 15368 - 16775 (+) CP000407.1:567875-569282 -
- SSU05_0578 ABP89544.1 17297 - 18314 (+) CP000407.1:569804-570821 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1452 (+) CDS No
- 1470 - 2159 (+) TC: gnl|TC-DB|Q93E03|8.A.3.2.3 Yes
- 2169 - 2864 (+) TC: gnl|TC-DB|Q93NW3|8.A.3.2.2 Yes
- 2890 - 3618 (+) other Yes
- 3643 - 4881 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 5056 - 6225 (+) CAZyme: GT4 Yes
- 6229 - 7386 (+) CAZyme: GT4 Yes
- 7700 - 9145 (+) CAZyme: GT2|GT2_Glyco_tranf_2_4 Yes
- 9180 - 10412 (+) other Yes
- 10551 - 11549 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 11542 - 12546 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 14389 - 15204 (+) CDS No
- 15244 - 15360 (+) CDS No
- 15369 - 16775 (+) CDS No
- 17298 - 18314 (+) CDS No

PUL ID

PUL0280

PubMed

23226442, PLoS One. 2012;7(11):e50987. doi: 10.1371/journal.pone.0050987. Epub 2012 Nov 30.

Title

HP0197 contributes to CPS synthesis and the virulence of Streptococcus suis via CcpA.

Author

Zhang A, Chen B, Yuan Z, Li R, Liu C, Zhou H, Chen H, Jin M

Abstract

Streptococcus suis serotype 2 (SS2), a major swine pathogen and an emerging zoonotic agent, has greatly challenged global public health. The encoding proteins with unknown functions the bacterium encodes are an obstruction to studies of the pathogenesis. A novel surface protective antigen HP0197 is one of these proteins which have no sequence homology to any known protein. In the present study, the protein was determined to be involved in bacterial virulence through an evaluation of the isogenic mutant (Deltahp0197) in both mice and pigs. The experimental infection also indicated that Deltahp0197 could be cleared easily during infection, which could be attributed to the reduced thickness of the capsular polysaccharides (CPS) and the significantly reduced phagocytotic resistance. Microarrays-based comparative transcriptome analysis suggested that the suppressed expression of the operon responsible for CPS synthesis might be reversed by CcpA activity, which controlled global regulation of carbon catabolite through the binding of the CcpA and HPr-Ser-46-P to the catabolite-responsive elements (cre) of the target operons. The hypothesis was approved by the fact that the purified FLAG-tagged HPr from WT stain exhibited a higher binding activity to cre with CcpA compared to the Deltahp0197 by the Electrophoretic Mobility Shift Assay, suggesting lower level of phosphorylation of the phosphocarrier protein HPr at residue Ser-46 (HPr-Ser-46P) in Deltahp0197. These indicated that HP0197 could enhance CcpA activity to control the expression of genes involved in carbohydrate utilization and CPS synthesis, thus contributing to the virulence of S. suis.