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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0283

PubMed

23199239, Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2.

Characterization method

microarray,high performance anion exchange chromatography,liquid chromatography and mass spectrometry

Genomic accession number

CP000303.1

Nucelotide position range

555379-565250

Substrate

galactooligosaccharide

Loci

bbr_0417-bbr_0422

Species

Bifidobacterium breve/1685

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Bbr_0417 ABE95115.1 0 - 1350 (+) CP000303.1:555379-556729 -
- Bbr_0418 ABE95116.1 1570 - 2533 (+) CP000303.1:556949-557912 -
- Bbr_0419 ABE95117.1 2585 - 3584 (+) CP000303.1:557964-558963 -
lacZ4 Bbr_0420 ABE95118.1 3751 - 5884 (+) CP000303.1:559130-561263 3.2.1.23
- Bbr_0421 ABE95119.1 5927 - 6983 (+) CP000303.1:561306-562362 -
galA Bbr_0422 ABE95120.1 7178 - 9872 (+) CP000303.1:562557-565251 3.2.1.89

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1350 (+) TC: gnl|TC-DB|P94528|3.A.1.1.34 Yes
- 1571 - 2533 (+) TC: gnl|TC-DB|D6ZW63|3.A.1.1.48 Yes
- 2586 - 3584 (+) TC: gnl|TC-DB|D6ZW62|3.A.1.1.48 Yes
lacZ4 3752 - 5884 (+) CAZyme: GH42 Yes
- 5928 - 6983 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
galA 7179 - 9872 (+) CAZyme: GH53|CBM61 Yes

PUL ID

PUL0283

PubMed

23199239, Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2.

Title

Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003.

Author

O'Connell Motherway M, Kinsella M, Fitzgerald GF, van Sinderen D

Abstract

Several prebiotics, such as inulin, fructo-oligosaccharides and galacto-oligosaccharides, are widely used commercially in foods and there is convincing evidence, in particular for galacto-oligosaccharides, that prebiotics can modulate the microbiota and promote bifidobacterial growth in the intestinal tract of infants and adults. In this study we describe the identification and functional characterization of the genetic loci responsible for the transport and metabolism of purified galacto-oligosaccharides (PGOS) by Bifidobacterium breve UCC2003. We further demonstrate that an extracellular endogalactanase specified by several B. breve strains, including B. breve UCC2003, is essential for partial degradation of PGOS components with a high degree of polymerization. These partially hydrolysed PGOS components are presumed to be transported into the bifidobacterial cell via various ABC transport systems and sugar permeases where they are further degraded to galactose and glucose monomers that feed into the bifid shunt. This work significantly advances our molecular understanding of bifidobacterial PGOS metabolism and its associated genetic machinery to utilize this prebiotic.