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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0287

PubMed

23082033, Microbiology (Reading). 2012 Dec;158(Pt 12):2917-2926. doi: 10.1099/mic.0.062919-0. Epub 2012 Oct 18.

Characterization method

RT-PCR

Genomic accession number

CP000410.2

Nucelotide position range

424599-432882

Substrate

unknown polysaccharide

Loci

SPD_0423-SPD_0428

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SPD_0423 ABJ55302.1 0 - 1224 (-) CP000410.2:424599-425823 -
- SPD_0424 ABJ54258.1 1391 - 2714 (+) CP000410.2:425990-427313 -
- SPD_0425 ABJ54107.1 2715 - 4602 (+) CP000410.2:427314-429201 -
lacF-1 SPD_0426 ABJ55198.1 4769 - 5114 (+) CP000410.2:429368-429713 2.7.1.-
lacG-1 SPD_0427 ABJ54778.1 5123 - 6536 (+) CP000410.2:429722-431135 3.2.1.85
lacE-1 SPD_0428 ABJ53919.1 6604 - 8284 (+) CP000410.2:431203-432883 2.7.1.-

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1224 (-) TF: DBD-Pfam|MarR,DBD-SUPERFAMILY|0040266 No
- 1392 - 2714 (+) TC: gnl|TC-DB|P46317|4.A.3.2.2 Yes
- 2716 - 4602 (+) other Yes
lacF-1 4770 - 5114 (+) TC: gnl|TC-DB|Q045X4|4.A.3.1.2 Yes
lacG-1 5124 - 6536 (+) CAZyme: GH1 Yes
lacE-1 6605 - 8284 (+) TC: gnl|TC-DB|P11162|4.A.3.1.1 Yes

PUL ID

PUL0287

PubMed

23082033, Microbiology (Reading). 2012 Dec;158(Pt 12):2917-2926. doi: 10.1099/mic.0.062919-0. Epub 2012 Oct 18.

Title

Characterization of the ROK-family transcriptional regulator RokA of Streptococcus pneumoniae D39.

Author

Shafeeq S, Kloosterman TG, Rajendran V, Kuipers OP

Abstract

The Gram-positive human pathogen Streptococcus pneumoniae possesses an unusually high number of gene clusters specific for carbohydrate utilization. This provides it with the ability to use a wide array of sugars, which may aid during infection and survival in different environmental conditions present in the host. In this study, the regulatory mechanism of transcription of a gene cluster, SPD0424-8, putatively encoding a cellobiose/lactose-specific phosphotransferase system is investigated. We demonstrate that this gene cluster is transcribed as one transcriptional unit directed by the promoter of the SPD0424 gene. Upstream of SPD0424, a gene was identified encoding a ROK-family transcriptional regulator (RokA: SPD0423). DNA microarray and transcriptional reporter analyses with a rokA mutant revealed that RokA acts as a transcriptional repressor of the SPD0424-8 operon. Furthermore, we identified a 25 bp AT-rich DNA operator site (5'-TATATTTAATTTATAAAAAATAAAA-3') in the promoter region of SPD0424, which was validated by promoter truncation studies, DNase I footprinting and electrophoretic mobility-shift assays. We tested a large range of different sugars for their effect on the expression of the SPD0424-8 operon, but only moderate variation in expression was observed in the conditions applied. Therefore, a co-factor for RokA-mediated transcriptional control could not be identified.