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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0348

PubMed

22449996, J Med Invest. 2012;59(1-2):79-94. doi: 10.2152/jmi.59.79.

Characterization method

enzyme activity assay

Genomic accession number

AP006841.1

Nucelotide position range

2022215-2049211

Substrate

sialoglycoconjugate

Loci

BF1729-BF1741

Species

Bacteroides fragilis/817

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BF1729 BAD48476.1 0 - 1635 (+) AP006841.1:2022215-2023850 -
- BF1730 BAD48477.1 1666 - 3679 (+) AP006841.1:2023881-2025894 -
- BF1731 BAD48478.1 3699 - 4362 (+) AP006841.1:2025914-2026577 -
- BF1732 BAD48479.1 4358 - 6431 (+) AP006841.1:2026573-2028646 -
- BF1733 BAD48480.1 6452 - 9023 (+) AP006841.1:2028667-2031238 -
- BF1734 BAD48481.1 9042 - 11367 (+) AP006841.1:2031257-2033582 -
- BF1735 BAD48482.1 11377 - 12592 (+) AP006841.1:2033592-2034807 -
- BF1736 BAD48483.1 12659 - 14732 (+) AP006841.1:2034874-2036947 -
- BF1737 BAD48484.1 14792 - 17042 (+) AP006841.1:2037007-2039257 -
- BF1738 BAD48485.1 17055 - 19557 (+) AP006841.1:2039270-2041772 -
- BF1739 BAD48486.1 19653 - 22398 (+) AP006841.1:2041868-2044613 -
- BF1740 BAD48487.1 22412 - 24044 (+) AP006841.1:2044627-2046259 -
- BF1741 BAD48488.1 24210 - 26997 (+) AP006841.1:2046425-2049212 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1635 (+) CAZyme: GH33 Yes
- 1667 - 3679 (+) CAZyme: GH20 Yes
- 3700 - 4362 (+) other Yes
- 4359 - 6431 (+) other Yes
- 6453 - 9023 (+) CAZyme: GH2 Yes
- 9043 - 11367 (+) CAZyme: CBM32|GH20 Yes
- 11378 - 12592 (+) other Yes
- 12660 - 14732 (+) CAZyme: GH20 Yes
- 14793 - 17042 (+) CAZyme: GH92 Yes
- 17056 - 19557 (+) CAZyme: GH2 Yes
- 19654 - 22398 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 22413 - 24044 (+) other Yes
- 24211 - 26997 (+) CAZyme: GH2 Yes

PUL ID

PUL0348

PubMed

22449996, J Med Invest. 2012;59(1-2):79-94. doi: 10.2152/jmi.59.79.

Title

Characterization of a gene cluster for sialoglycoconjugate utilization in Bacteroides fragilis.

Author

Nakayama-Imaohji H, Ichimura M, Iwasa T, Okada N, Ohnishi Y, Kuwahara T

Abstract

Recent analysis of the whole genome sequence of Bacteroides fragilis revealed extensive duplication of polysaccharide utilization genes in this anaerobe. Here we analyzed a unique 27-kb gene cluster (sgu) comprised of the 13 sialoglycoconjugates-utilization genes, which include the sialidase gene (nanH1) in B. fragilis strain YCH46. The genes were tightly organized and transcribed polycistronically. Comparative PCR scanning demonstrated that the sgu locus was conserved among the Bacteroides strains tested. Based on the transcriptional profiles generated by reverse transcriptase PCR, the sgu locus can be classified into at least three regulatory units: 1) sialic acid- or sialooligosaccharide-inducible genes, 2) constitutively expressed genes that can be down-regulated by catabolite repression, and 3) constitutively expressed genes. In vitro comparison of the growth of a sgu locus deletion mutant (SGUM172941) with a wild type strain indicates that this locus is necessary for B. fragilis to efficiently utilize mucin as a carbon source. Furthermore, SGUM172941 was defective in colonization of the intestines of germ-free mice under competitive conditions. These data indicate that the sgu locus in B. fragilis plays a crucial role in the utilization of host-derived sialoglycoconjugates and the stable colonization of this anaerobe in the human gut.