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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0349

PubMed

22230464, Mol Oral Microbiol. 2012 Feb;27(1):34-44. doi: 10.1111/j.2041-1014.2011.00627.x. Epub 2011 Oct 4.

Characterization method

microarray

Genomic accession number

NC_013192.1

Nucelotide position range

1609965-1613940

Substrate

sucrose

Loci

LEBU_RS07530-LEBU_RS07540

Species

Leptotrichia buccalis/40542

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- LEBU_RS07530 WP_015769739.1 0 - 1323 (-) NC_013192.1:1609965-1611288 -
- LEBU_RS07535 WP_015769740.1 1549 - 2257 (+) NC_013192.1:1611514-1612222 -
- LEBU_RS07540 WP_015769741.1 2386 - 3976 (-) NC_013192.1:1612351-1613941 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1323 (-) CAZyme: GH4 Yes
- 1550 - 2257 (+) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767 Yes
- 2387 - 3976 (-) TC: gnl|TC-DB|C7NB69|4.A.1.1.16 Yes

PUL ID

PUL0349

PubMed

22230464, Mol Oral Microbiol. 2012 Feb;27(1):34-44. doi: 10.1111/j.2041-1014.2011.00627.x. Epub 2011 Oct 4.

Title

Metabolism of sugars by genetically diverse species of oral Leptotrichia.

Author

Thompson J, Pikis A

Abstract

Leptotrichia buccalis ATCC 14201 is a gram-negative, anaerobic rod-shaped bacterium resident in oral biofilm at the tooth surface. The sequenced genome of this organism reveals three contiguous genes at loci: Lebu_1525, Lebu_1526 and Lebu_1527. The translation products of these genes exhibit significant homology with phospho-alpha-glucosidase (Pagl), a regulatory protein (GntR) and a phosphoenol pyruvate-dependent sugar transport protein (EIICB), respectively. In non-oral bacterial species, these genes comprise the sim operon that facilitates sucrose isomer metabolism. Growth studies showed that L. buccalis fermented a wide variety of carbohydrates, including four of the five isomers of sucrose. Growth on the isomeric disaccharides elicited expression of a 50-kDa polypeptide comparable in size to that encoded by Lebu_1525. The latter gene was cloned, and the expressed protein was purified to homogeneity from Escherichia coli TOP10 cells. In the presence of two cofactors, NAD(+) and Mn(2+) ions, the enzyme readily hydrolyzed p-nitrophenyl-alpha-glucopyranoside 6-phosphate (pNPalphaG6P), a chromogenic analogue of the phosphorylated isomers of sucrose. By comparative sequence alignment, immunoreactivity and signature motifs, the enzyme can be assigned to the phospho-alpha-glucosidase (Pagl) clade of Family 4 of the glycosyl hydrolase super family. We suggest that the products of Lebu_1527 and Lebu_1525, catalyze the phosphorylative translocation and hydrolysis of sucrose isomers in L. buccalis, respectively. Four genetically diverse, but 16S rDNA-related, species of Leptotrichia have recently been described: L. goodfellowii, L. hofstadii, L. shahii and L. wadei. The phenotypic traits of these new species, with respect to carbohydrate utilization, have also been determined.