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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0362

PubMed

17311090, PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.

Characterization method

enzyme activity assay

Genomic accession number

AE008922.1

Nucelotide position range

3996974-4004221

Substrate

sucrose

Loci

XCC3356-XCC3359

Species

Xanthomonas campestris pv. campestris/340

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

cebR XCC3356 AAM42626.1 0 - 1032 (+) AE008922.1:3996974-3998006 -
suc1 XCC3357 AAM42627.1 1259 - 2579 (-) AE008922.1:3998233-3999553 -
fyuA XCC3358 AAM42628.1 2826 - 5301 (+) AE008922.1:3999800-4002275 -
- XCC3359 AAM42629.1 5334 - 7248 (+) AE008922.1:4002308-4004222 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

cebR 1 - 1032 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
suc1 1260 - 2579 (-) TC: gnl|TC-DB|Q9A612|2.A.2.6.2 Yes
fyuA 2827 - 5301 (+) other Yes
- 5335 - 7248 (+) CAZyme: GH13_4 Yes

PUL ID

PUL0362

PubMed

17311090, PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.

Title

Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria.

Author

Blanvillain S, Meyer D, Boulanger A, Lautier M, Guynet C, Denance N, Vasse J, Lauber E, Arlat M

Abstract

TonB-dependent receptors (TBDRs) are outer membrane proteins mainly known for the active transport of iron siderophore complexes in Gram-negative bacteria. Analysis of the genome of the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), predicts 72 TBDRs. Such an overrepresentation is common in Xanthomonas species but is limited to only a small number of bacteria. Here, we show that one Xcc TBDR transports sucrose with a very high affinity, suggesting that it might be a sucrose scavenger. This TBDR acts with an inner membrane transporter, an amylosucrase and a regulator to utilize sucrose, thus defining a new type of carbohydrate utilization locus, named CUT locus, involving a TBDR for the transport of substrate across the outer membrane. This sucrose CUT locus is required for full pathogenicity on Arabidopsis, showing its importance for the adaptation to host plants. A systematic analysis of Xcc TBDR genes and a genome context survey suggested that several Xcc TBDRs belong to other CUT loci involved in the utilization of various plant carbohydrates. Interestingly, several Xcc TBDRs and CUT loci are conserved in aquatic bacteria such as Caulobacter crescentus, Colwellia psychrerythraea, Saccharophagus degradans, Shewanella spp., Sphingomonas spp. or Pseudoalteromonas spp., which share the ability to degrade a wide variety of complex carbohydrates and display TBDR overrepresentation. We therefore propose that TBDR overrepresentation and the presence of CUT loci designate the ability to scavenge carbohydrates. Thus CUT loci, which seem to participate to the adaptation of phytopathogenic bacteria to their host plants, might also play a very important role in the biogeochemical cycling of plant-derived nutrients in marine environments. Moreover, the TBDRs and CUT loci identified in this study are clearly different from those characterized in the human gut symbiont Bacteroides thetaiotaomicron, which allow glycan foraging, suggesting a convergent evolution of TBDRs in Proteobacteria and Bacteroidetes.