17311090, PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.

Characterization method

enzyme activity assay

Genomic accession number


Nucelotide position range







Xanthomonas campestris pv. campestris/340

Degradation or Biosynthesis


Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

xynA XCC4115 AAM43336.1 0 - 1158 (-) AE008922.1:4895164-4896322 -
- XCC4116 AAM43337.1 1298 - 4190 (-) AE008922.1:4896462-4899354 -
hrmI XCC4117 AAM43338.1 4275 - 5691 (-) AE008922.1:4899439-4900855 -
xynB XCC4118 AAM43339.1 5742 - 6735 (-) AE008922.1:4900906-4901899 -
exuT XCC4119 AAM43340.1 6744 - 8250 (-) AE008922.1:4901908-4903414 -
cirA XCC4120 AAM43341.1 8593 - 11476 (+) AE008922.1:4903757-4906640 -
xylP XCC4121 AAM43342.1 11573 - 13061 (+) AE008922.1:4906737-4908225 -
xsa XCC4122 AAM43343.1 13096 - 14131 (+) AE008922.1:4908260-4909295 -

Cluster number


Gene name

Gene position

Gene type

Found by CGCFinder?

xynA 1 - 1158 (-) CAZyme: CBM22|GH10 Yes
- 1299 - 4190 (-) CAZyme: GH2 Yes
hrmI 4276 - 5691 (-) other Yes
xynB 5743 - 6735 (-) CAZyme: GH10 Yes
exuT 6745 - 8250 (-) TC: gnl|TC-DB|Q07YH1|2.A.1.14.25 Yes
cirA 8594 - 11476 (+) other Yes
xylP 11574 - 13061 (+) TC: gnl|TC-DB|A1S5F2|2.A.2.3.6 Yes
xsa 13097 - 14131 (+) CAZyme: GH43_1 Yes




17311090, PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.


Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria.


Blanvillain S, Meyer D, Boulanger A, Lautier M, Guynet C, Denance N, Vasse J, Lauber E, Arlat M


TonB-dependent receptors (TBDRs) are outer membrane proteins mainly known for the active transport of iron siderophore complexes in Gram-negative bacteria. Analysis of the genome of the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), predicts 72 TBDRs. Such an overrepresentation is common in Xanthomonas species but is limited to only a small number of bacteria. Here, we show that one Xcc TBDR transports sucrose with a very high affinity, suggesting that it might be a sucrose scavenger. This TBDR acts with an inner membrane transporter, an amylosucrase and a regulator to utilize sucrose, thus defining a new type of carbohydrate utilization locus, named CUT locus, involving a TBDR for the transport of substrate across the outer membrane. This sucrose CUT locus is required for full pathogenicity on Arabidopsis, showing its importance for the adaptation to host plants. A systematic analysis of Xcc TBDR genes and a genome context survey suggested that several Xcc TBDRs belong to other CUT loci involved in the utilization of various plant carbohydrates. Interestingly, several Xcc TBDRs and CUT loci are conserved in aquatic bacteria such as Caulobacter crescentus, Colwellia psychrerythraea, Saccharophagus degradans, Shewanella spp., Sphingomonas spp. or Pseudoalteromonas spp., which share the ability to degrade a wide variety of complex carbohydrates and display TBDR overrepresentation. We therefore propose that TBDR overrepresentation and the presence of CUT loci designate the ability to scavenge carbohydrates. Thus CUT loci, which seem to participate to the adaptation of phytopathogenic bacteria to their host plants, might also play a very important role in the biogeochemical cycling of plant-derived nutrients in marine environments. Moreover, the TBDRs and CUT loci identified in this study are clearly different from those characterized in the human gut symbiont Bacteroides thetaiotaomicron, which allow glycan foraging, suggesting a convergent evolution of TBDRs in Proteobacteria and Bacteroidetes.