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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0366

PubMed

10931310, Mol Microbiol. 2000 Jul;37(1):125-35. doi: 10.1046/j.1365-2958.2000.01969.x.

Characterization method

RT-PCR

Genomic accession number

AF228498.1

Nucelotide position range

33-11506

Substrate

N-acetyl-D-galactosamine,d-galactosamine

Loci

agaR-kbaZ-agaVWEFAS-kbaY-agaBCDEI

Species

Escherichia coli/562

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

agaR - AAF81081.1 33 - 843 (-) AF228498.1:66-876 -
kbaZ - AAF81082.1 1091 - 2372 (+) AF228498.1:1124-2405 -
agaV - AAF81083.1 2358 - 2868 (+) AF228498.1:2391-2901 -
agaW - AAF81084.1 2878 - 3658 (+) AF228498.1:2911-3691 -
agaE - AAF81085.1 3647 - 4526 (+) AF228498.1:3680-4559 -
agaF - AAF81086.1 4543 - 4978 (+) AF228498.1:4576-5011 -
agaA - AAF81087.1 4953 - 6108 (+) AF228498.1:4986-6141 -
agaS - AAF81088.1 6456 - 7611 (+) AF228498.1:6489-7644 -
kbaY - AAF81089.1 7623 - 8484 (+) AF228498.1:7656-8517 -
agaB - AAF81090.1 8650 - 9127 (+) AF228498.1:8683-9160 -
agaC - AAF81091.1 9165 - 9969 (+) AF228498.1:9198-10002 -
agaD - AAF81092.1 9958 - 10750 (+) AF228498.1:9991-10783 -
agaI - AAF81093.1 10750 - 11506 (+) AF228498.1:10783-11539 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 34 - 843 (-) TF: DBD-Pfam|HTH_DeoR,DBD-SUPERFAMILY|0043758 No
- 1092 - 2372 (+) CDS No
- 2359 - 2868 (+) TC: gnl|TC-DB|Q9KIQ4|4.A.6.1.4 Yes
- 2879 - 3658 (+) TC: gnl|TC-DB|Q9KIQ3|4.A.6.1.4 Yes
- 3648 - 4526 (+) TC: gnl|TC-DB|Q9KIQ2|4.A.6.1.4 Yes
- 4544 - 4978 (+) TC: gnl|TC-DB|Q8Y8V8|4.A.6.1.16 Yes
- 4954 - 6108 (+) CAZyme: CE9 Yes
- 6457 - 7611 (+) STP: STP|SIS,STP|SIS Yes
- 7624 - 8484 (+) other Yes
- 8651 - 9127 (+) TC: gnl|TC-DB|P42909|4.A.6.1.5 Yes
- 9166 - 9969 (+) TC: gnl|TC-DB|Q9KIP7|4.A.6.1.5 Yes
- 9959 - 10750 (+) TC: gnl|TC-DB|P42911|4.A.6.1.5 Yes
- 10751 - 11506 (+) CDS No

PUL ID

PUL0366

PubMed

10931310, Mol Microbiol. 2000 Jul;37(1):125-35. doi: 10.1046/j.1365-2958.2000.01969.x.

Title

Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.

Author

Brinkkotter A, Kloss H, Alpert C, Lengeler JW

Abstract

Among enteric bacteria, the ability to grow on N-acetyl-galactosamine (GalNAc or Aga) and on D-galactosamine (GalN or Gam) differs. Thus, strains B, C and EC3132 of Escherichia coli are Aga+ Gam+ whereas E. coli K-12 is Aga- Gam-, similarly to Klebsiella pneumoniae KAY2026, Klebsiella oxytoca M5a1 and Salmonella typhimurium LT2. The former strains carry a complete aga/kba gene cluster at 70.5 min of their gene map. These genes encode an Aga-specific phosphotransferase system (PTS) or IIAga (agaVWE) and a GalN-specific PTS or IIGam (agaBCD). Both PTSs belong to the mannose-sorbose family, i.e. the IIB, IIC and IID domains are encoded by different genes, and they share a IIA domain (agaF). Furthermore, the genes encode an Aga6P-deacetylase (agaA), a GalN6P deaminase (agaI), a tagatose-bisphosphate aldolase comprising two different peptides (kbaYZ) and a putative isomerase (agaS), i.e. complete pathways for the transport and degradation of both amino sugars. The genes are organized in two adjacent operons (kbaZagaVWEFA and agaS kbaYagaBCDI) and controlled by a repressor AgaR. Its gene agaR is located upstream of kbaZ, and AgaR responds to GalNAc and GalN in the medium. All Aga- Gam- strains, however, carry a deletion covering genes agaW' EF 'A; consequently they lack active IIAga and IIGam PTSs, thus explaining their inability to grow on the two amino sugars. Remnants of a putative recombination site flank the deleted DNA in the various Aga- Gam- enteric bacteria. Derivatives with an Aga+ Gam- phenotype can be isolated from E. coli K-12. These retain the DeltaagaW' EF 'A deletion and carry suppressor mutations in the gat and nag genes for galactitol and N-acetyl-glucosamine metabolism, respectively, that allow growth on Aga but not on GalN.