PUL ID

PUL0368

PubMed

19033196, Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24.

Characterization method

microarray, Western Blot

Genomic accession number

CP001095.1

Nucelotide position range

2595574-2636747

Substrate

human milk oligosaccharide

Loci

Blon_2332-Blon_2361

Species

Bifidobacterium longum subsp. infantis/1682

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Blon_2332 ACJ53390.1 0 - 1533 (-) CP001095.1:2595574-2597107 -
- Blon_2333 ACJ53391.1 1633 - 2989 (-) CP001095.1:2597207-2598563 -
- Blon_2334 ACJ53392.1 3284 - 6356 (+) CP001095.1:2598858-2601930 -
- Blon_2335 ACJ53393.1 6532 - 8881 (-) CP001095.1:2602106-2604455 -
- Blon_2336 ACJ53394.1 8912 - 10349 (-) CP001095.1:2604486-2605923 -
- Blon_2337 ACJ53395.1 10370 - 10808 (-) CP001095.1:2605944-2606382 -
- Blon_2338 ACJ53396.1 10844 - 11741 (-) CP001095.1:2606418-2607315 -
- Blon_2339 ACJ53397.1 11884 - 12676 (-) CP001095.1:2607458-2608250 -
- Blon_2340 ACJ53398.1 12695 - 13973 (-) CP001095.1:2608269-2609547 -
- Blon_2341 ACJ53399.1 14123 - 14783 (-) CP001095.1:2609697-2610357 -
- Blon_2342 ACJ53400.1 14922 - 15885 (-) CP001095.1:2610496-2611459 -
- Blon_2343 ACJ53401.1 15903 - 16857 (-) CP001095.1:2611477-2612431 -
- Blon_2344 ACJ53402.1 16943 - 18536 (-) CP001095.1:2612517-2614110 -
- Blon_2345 ACJ53403.1 18738 - 19701 (-) CP001095.1:2614312-2615275 -
- Blon_2346 ACJ53404.1 19719 - 20673 (-) CP001095.1:2615293-2616247 -
- Blon_2347 ACJ53405.1 20759 - 22355 (-) CP001095.1:2616333-2617929 -
- Blon_2348 ACJ53406.1 22489 - 23674 (-) CP001095.1:2618063-2619248 3.2.1.18
- Blon_2349 ACJ53407.1 23839 - 24796 (-) CP001095.1:2619413-2620370 -
- Blon_2350 ACJ53408.1 24977 - 26570 (-) CP001095.1:2620551-2622144 -
- Blon_2351 ACJ53409.1 26946 - 28551 (-) CP001095.1:2622520-2624125 -
- Blon_2352 ACJ53410.1 28938 - 30537 (-) CP001095.1:2624512-2626111 -
- Blon_2353 ACJ53411.1 30690 - 30813 (-) CP001095.1:2626264-2626387 -
- Blon_2354 ACJ53412.1 30989 - 32585 (-) CP001095.1:2626563-2628159 -
- Blon_2355 ACJ53413.1 32740 - 34696 (-) CP001095.1:2628314-2630270 -
- Blon_2356 ACJ53414.1 34792 - 35533 (-) CP001095.1:2630366-2631107 -
- Blon_2357 ACJ53415.1 35566 - 36859 (-) CP001095.1:2631140-2632433 -
- Blon_2358 ACJ53416.1 36988 - 38308 (-) CP001095.1:2632562-2633882 -
- Blon_2359 ACJ53417.1 38291 - 39110 (-) CP001095.1:2633865-2634684 -
- Blon_2360 ACJ53418.1 39106 - 40054 (-) CP001095.1:2634680-2635628 -
- Blon_2361 ACJ53419.1 40055 - 41174 (-) CP001095.1:2635629-2636748 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1533 (-) TC: gnl|TC-DB|Q9X761|2.A.2.2.3 Yes
- 1634 - 2989 (-) TF: DBD-SUPERFAMILY|0039939 Yes
- 3285 - 6356 (+) CAZyme: GH2 Yes
- 6533 - 8881 (-) CAZyme: GH95 Yes
- 8913 - 10349 (-) CAZyme: GH29 Yes
- 10371 - 10808 (-) other Yes
- 10845 - 11741 (-) other Yes
- 11885 - 12676 (-) other Yes
- 12696 - 13973 (-) other Yes
- 14124 - 14783 (-) TC: gnl|TC-DB|M4RG66|9.B.28.1.10 Yes
- 14923 - 15885 (-) TC: gnl|TC-DB|A9QDR8|3.A.1.1.29 Yes
- 15904 - 16857 (-) TC: gnl|TC-DB|Q9KWT8|3.A.1.1.10 Yes
- 16944 - 18536 (-) STP: STP|SBP_bac_1 Yes
- 18739 - 19701 (-) TC: gnl|TC-DB|A9QDR8|3.A.1.1.29 Yes
- 19720 - 20673 (-) TC: gnl|TC-DB|Q9KWT8|3.A.1.1.10 Yes
- 20760 - 22355 (-) STP: STP|SBP_bac_1 Yes
- 22490 - 23674 (-) CAZyme: GH33 Yes
- 23840 - 24796 (-) other Yes
- 24978 - 26570 (-) STP: STP|SBP_bac_1 Yes
- 26947 - 28551 (-) STP: STP|SBP_bac_1 Yes
- 28939 - 30537 (-) STP: STP|SBP_bac_1 Yes
- 30691 - 30813 (-) other Yes
- 30990 - 32585 (-) STP: STP|SBP_bac_1 Yes
- 32741 - 34696 (-) CAZyme: GH20 Yes
- 34793 - 35533 (-) other Yes
- 35567 - 36859 (-) STP: STP|SBP_bac_1 Yes
- 36989 - 38308 (-) other Yes
- 38292 - 39110 (-) TC: gnl|TC-DB|Q93J92|3.A.1.1.36 Yes
- 39107 - 40054 (-) TC: gnl|TC-DB|P29823|3.A.1.1.4 Yes
- 40056 - 41174 (-) TC: gnl|TC-DB|Q9X103|3.A.1.1.22 Yes

PUL ID

PUL0368

PubMed

19033196, Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24.

Title

The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome.

Author

Sela DA, Chapman J, Adeuya A, Kim JH, Chen F, Whitehead TR, Lapidus A, Rokhsar DS, Lebrilla CB, German JB, Price NP, Richardson PM, Mills DA

Abstract

Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated by bifidobacteria. Accordingly, the complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects a competitive nutrient-utilization strategy targeting milk-borne molecules which lack a nutritive value to the neonate. Several chromosomal loci reflect potential adaptation to the infant host including a 43 kbp cluster encoding catabolic genes, extracellular solute binding proteins and permeases predicted to be active on milk oligosaccharides. An examination of in vivo metabolism has detected the hallmarks of milk oligosaccharide utilization via the central fermentative pathway using metabolomic and proteomic approaches. Finally, conservation of gene clusters in multiple isolates corroborates the genomic mechanism underlying milk utilization for this infant-associated phylotype.