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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0370

PubMed

19628558, Microbiology (Reading). 2009 Nov;155(Pt 11):3652-3660. doi: 10.1099/mic.0.029496-0. Epub 2009 Jul 23.

Characterization method

RT-PCR

Genomic accession number

AP009044.1

Nucelotide position range

3017544-3021779

Substrate

beta-glucoside

Loci

Cgr_2727-Cgr_2729

Species

Corynebacterium glutamicum/1718

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- cgR_2727 BAF55743.1 0 - 870 (-) AP009044.1:3017544-3018414 -
- cgR_2728 BAF55745.1 909 - 2319 (-) AP009044.1:3018453-3019863 -
- cgR_2729 BAF55746.1 2379 - 4236 (-) AP009044.1:3019923-3021780 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 870 (-) CDS No
- 910 - 2319 (-) CAZyme: GH1 Yes
- 2380 - 4236 (-) TC: gnl|TC-DB|Q8GGK3|4.A.1.2.5 Yes

PUL ID

PUL0370

PubMed

19628558, Microbiology (Reading). 2009 Nov;155(Pt 11):3652-3660. doi: 10.1099/mic.0.029496-0. Epub 2009 Jul 23.

Title

Identification of a second beta-glucoside phosphoenolpyruvate: carbohydrate phosphotransferase system in Corynebacterium glutamicum R.

Author

Tanaka Y, Teramoto H, Inui M, Yukawa H

Abstract

The phosphoenolpyruvate : carbohydrate phosphotransferase system (PTS) catalyses carbohydrate transport by coupling it to phosphorylation. Previously, we reported a Corynebacterium glutamicum R beta-glucoside PTS encoded by bglF. Here we report that C. glutamicum R contains an additional beta-glucoside PTS gene, bglF2, organized in a cluster with a putative phospho-beta-glucosidase gene, bglA2, and a putative antiterminator, bglG2. While single gene disruption strains of either bglF or bglF2 were able to utilize salicin or arbutin as sole carbon sources, a double disruption strain exhibited defects in utilization of both carbon sources. Expression of both bglF and bglF2 was induced in the presence of either salicin or arbutin, although disruption of bglG2 affected only bglF2 expression. Moreover, in the presence of either salicin or arbutin, glucose completely repressed the expression of bglF but only slightly repressed that of bglF2. We conclude that BglF and BglF2 have a redundant role in beta-glucoside transport even though the catabolite repression control of their encoding genes is different. We also show that expression of both bglF and bglF2 requires the general PTS.