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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0377

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

4269304-4322090

Substrate

glycosaminoglycan

Loci

BT_3324-BT_3350

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_3324 AAO78430.1 0 - 3045 (+) AE015928.1:4269304-4272349 -
- BT_3325 AAO78431.1 3839 - 5069 (-) AE015928.1:4273143-4274373 -
- BT_3326 AAO78432.1 5155 - 6100 (-) AE015928.1:4274459-4275404 -
- BT_3327 AAO78433.1 6127 - 7585 (-) AE015928.1:4275431-4276889 -
- BT_3328 AAO78434.1 7765 - 10372 (-) AE015928.1:4277069-4279676 -
- BT_3329 AAO78435.1 10385 - 12323 (-) AE015928.1:4279689-4281627 -
- BT_3330 AAO78436.1 12361 - 13405 (-) AE015928.1:4281665-4282709 -
- BT_3331 AAO78437.1 13438 - 15157 (-) AE015928.1:4282742-4284461 -
- BT_3332 AAO78438.1 15197 - 18359 (-) AE015928.1:4284501-4287663 -
- BT_3333 AAO78439.1 18518 - 20054 (-) AE015928.1:4287822-4289358 -
- BT_3334 AAO78440.1 20353 - 24418 (-) AE015928.1:4289657-4293722 -
- BT_3335 AAO78441.1 24529 - 25171 (+) AE015928.1:4293833-4294475 -
- BT_3336 AAO78442.1 25231 - 26002 (-) AE015928.1:4294535-4295306 -
- BT_3337 AAO78443.1 26020 - 27406 (-) AE015928.1:4295324-4296710 -
- BT_3338 AAO78444.1 27417 - 30633 (-) AE015928.1:4296721-4299937 -
- BT_3339 AAO78445.1 30649 - 31825 (-) AE015928.1:4299953-4301129 -
- BT_3340 AAO78446.1 32004 - 35115 (-) AE015928.1:4301308-4304419 -
- BT_3341 AAO78447.1 35265 - 37194 (+) AE015928.1:4304569-4306498 -
- BT_3342 AAO78448.1 37234 - 38014 (+) AE015928.1:4306538-4307318 -
- BT_3343 AAO78449.1 38019 - 38424 (-) AE015928.1:4307323-4307728 -
- BT_3344 AAO78450.1 38729 - 39740 (-) AE015928.1:4308033-4309044 -
- BT_3345 AAO78451.1 39768 - 41808 (-) AE015928.1:4309072-4311112 -
- BT_3346 AAO78452.1 41830 - 44914 (-) AE015928.1:4311134-4314218 -
- BT_3347 AAO78453.1 44937 - 46347 (-) AE015928.1:4314241-4315651 -
- BT_3348 AAO78454.1 46951 - 48154 (+) AE015928.1:4316255-4317458 -
- BT_3349 AAO78455.1 48260 - 49787 (+) AE015928.1:4317564-4319091 -
- BT_3350 AAO78456.1 49925 - 52787 (+) AE015928.1:4319229-4322091 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3045 (+) CAZyme: PL8_2|PL8 Yes
- 3840 - 5069 (-) other Yes
- 5156 - 6100 (-) other Yes
- 6128 - 7585 (-) other Yes
- 7766 - 10372 (-) CAZyme: PL29 Yes
- 10386 - 12323 (-) other Yes
- 12362 - 13405 (-) other Yes
- 13439 - 15157 (-) TC: gnl|TC-DB|C6Y217|8.A.46.1.3 Yes
- 15198 - 18359 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 18519 - 20054 (-) other Yes
- 20354 - 24418 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0037176,DBD-SUPERFAMILY|0035607 Yes
- 24530 - 25171 (+) other Yes
- 25232 - 26002 (-) other Yes
- 26021 - 27406 (-) TC: gnl|TC-DB|Q8ZRH0|2.A.6.2.25 Yes
- 27418 - 30633 (-) TC: gnl|TC-DB|Q8RTE4|2.A.6.2.22 Yes
- 30650 - 31825 (-) TC: gnl|TC-DB|P52477|2.A.6.2.6 Yes
- 32005 - 35115 (-) CAZyme: GH2 Yes
- 35266 - 37194 (+) other Yes
- 37235 - 38014 (+) other Yes
- 38020 - 38424 (-) other Yes
- 38730 - 39740 (-) other Yes
- 39769 - 41808 (-) other Yes
- 41831 - 44914 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 44938 - 46347 (-) other Yes
- 46952 - 48154 (+) CAZyme: GH88 Yes
- 48261 - 49787 (+) other Yes
- 49926 - 52787 (+) CAZyme: PL8_2 Yes

PUL ID

PUL0377

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.