PUL ID

PUL0407

PubMed

9066115, FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x.

Characterization method

primer extension analysis, enzyme activity assay

Genomic accession number

EU670680.1

Nucelotide position range

425-5007

Substrate

lactose

Loci

lacTEGF

Species

Lactobacillus casei/1582

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

lacT - ACG55690.1 15 - 894 (+) EU670680.1:440-1319 -
lacE - ACG55691.1 1014 - 2748 (+) EU670680.1:1439-3173 -
lacG - ACG55692.1 2774 - 4199 (+) EU670680.1:3199-4624 -
lacF - ACG55693.1 4244 - 4583 (+) EU670680.1:4669-5008 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 16 - 894 (+) CDS No
- 1015 - 2748 (+) TC: gnl|TC-DB|Q045H3|4.A.3.1.3 Yes
- 2775 - 4199 (+) CAZyme: GH1 Yes
- 4245 - 4583 (+) TC: gnl|TC-DB|Q045H4|4.A.3.1.3 Yes

PUL ID

PUL0407

PubMed

9066115, FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x.

Title

Establishing a model to study the regulation of the lactose operon in Lactobacillus casei.

Author

Gosalbes MJ, Monedero V, Alpert CA, Perez-Martinez G

Abstract

The chromosomally encoded lactose-specific phosphoenol pyruvate-dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15-] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15-] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.