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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0410

PubMed

21545489, FEMS Microbiol Lett. 2011 Jul;320(2):135-41. doi: 10.1111/j.1574-6968.2011.02300.x. Epub 2011 May 31.

Characterization method

RT-qPCR

Genomic accession number

AOMX01000002.1

Nucelotide position range

934681-941265

Substrate

sialic acid

Loci

H260_21035-H260_21065

Species

Escherichia coli/562

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- H260_21035 PJR32594.1 0 - 1050 (+) AOMX01000002.1:934681-935731 -
- H260_21040 PJR32595.1 1109 - 1574 (-) AOMX01000002.1:935790-936255 -
- H260_21045 PJR32596.1 1570 - 2446 (-) AOMX01000002.1:936251-937127 2.7.1.60
- H260_21050 PJR32597.1 2442 - 3132 (-) AOMX01000002.1:937123-937813 5.1.3.9
- H260_21055 PJR32598.1 3179 - 4670 (-) AOMX01000002.1:937860-939351 -
- H260_21060 PJR32599.1 4778 - 5672 (-) AOMX01000002.1:939459-940353 4.1.3.3
- H260_21065 PJR32600.1 5793 - 6585 (-) AOMX01000002.1:940474-941266 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1050 (+) CDS No
- 1110 - 1574 (-) CDS No
- 1571 - 2446 (-) CDS No
- 2443 - 3132 (-) CDS No
- 3180 - 4670 (-) TC: gnl|TC-DB|P41036|2.A.1.12.1 Yes
- 4779 - 5672 (-) CAZyme: GH33 Yes
- 5794 - 6585 (-) STP: STP|GntR No

PUL ID

PUL0410

PubMed

21545489, FEMS Microbiol Lett. 2011 Jul;320(2):135-41. doi: 10.1111/j.1574-6968.2011.02300.x. Epub 2011 May 31.

Title

Growth temperature regulation of some genes that define the superficial capsular carbohydrate composition of Escherichia coli K92.

Author

Navasa N, Rodriguez-Aparicio LB, Ferrero MA, Moteagudo-Mera A, Martinez-Blanco H

Abstract

We studied growth temperature as a factor controlling the expression of genes involved in capsular polymers of Escherichia coli K92. These genes are shown to be regulated by growth temperature. Expression levels of genes belonging to the kps cluster, responsible for polysialic acid (PA) biosynthesis, were significantly increased at 37 degrees C compared with at 19 degrees C, being up to 500-fold increased for neuE and neuS genes. Similarly, the genes for the nan operon, responsible for PA catabolism, also reached higher expression levels at 37 degrees C, although with slightly lower values (39-141-fold). In contrast, genes of the cps operon, which are implicated in colanic acid (CA) metabolism, were upregulated when the bacteria were grown at 19 degrees C, albeit to a much lesser extent (around twofold). This different regulation of genes involved in the biosynthesis of polysialic and CAs correlates with the reported maximal production temperatures for the two polymers. The results suggest that the metabolism of PA is predominantly regulated by changes in gene expression, while CA production may be regulated mainly by post-transcriptional processes such as phosphorylation-dephosphorylation reactions.