PUL ID

PUL0449

PubMed

28672166, Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.

Characterization method

sequence homology analysis, sugar utilization assay, NMR

Genomic accession number

KY574598

Nucelotide position range

1-15691

Substrate

O-antigen

Loci

ARO73527.1-ARO73539.1

Species

Escherichia albertii/208962

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - ARO73527.1 0 - 894 (+) KY574598.1:1-895 -
- - ARO73528.1 1428 - 2490 (+) KY574598.1:1429-2491 -
- - ARO73529.1 2676 - 3840 (+) KY574598.1:2677-3841 -
- - ARO73530.1 4690 - 6205 (+) KY574598.1:4691-6206 -
- - ARO73531.1 6186 - 7356 (+) KY574598.1:6187-7357 -
- - ARO73532.1 7352 - 8438 (+) KY574598.1:7353-8439 -
- - ARO73533.1 8444 - 9458 (+) KY574598.1:8445-9459 -
- - ARO73534.1 9470 - 10340 (+) KY574598.1:9471-10341 -
- - ARO73535.1 10329 - 10797 (+) KY574598.1:10330-10798 -
- - ARO73536.1 10831 - 11629 (+) KY574598.1:10832-11630 -
- - ARO73537.1 11631 - 12750 (+) KY574598.1:11632-12751 -
- - ARO73538.1 12869 - 14276 (+) KY574598.1:12870-14277 -
- - ARO73539.1 14524 - 15691 (+) KY574598.1:14525-15692 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 894 (+) CDS No
- 1429 - 2490 (+) CDS No
- 2677 - 3840 (+) CAZyme: GT4 Yes
- 4691 - 6205 (+) other Yes
- 6187 - 7356 (+) other Yes
- 7353 - 8438 (+) other Yes
- 8445 - 9458 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 9471 - 10340 (+) other Yes
- 10330 - 10797 (+) other Yes
- 10832 - 11629 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 11632 - 12750 (+) CAZyme: GT4 Yes
- 12870 - 14276 (+) CDS No
- 14525 - 15691 (+) CDS No

PUL ID

PUL0449

PubMed

28672166, Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.

Title

Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7.

Author

Naumenko OI, Zheng H, Senchenkova SN, Wang H, Li Q, Shashkov AS, Wang J, Knirel YA, Xiong Y

Abstract

The O-specific polysaccharides (OPSs) called O-antigens were obtained by mild acid degradation of the lipopolysaccharides of Escherichia albertii serotypes O3, O4, O6, and O7 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure was established for the OPS of E. albertii O4, which, to our knowledge, is unique among known bacterial polysaccharide structures: -->2)-alpha-l-Rhap-(1 --> 2)-alpha-l-Fucp-(1 --> 2)-beta-d-Galp-(1 --> 3)-alpha-d-GalpNAc-(1 --> 3)-beta-d-GlcpNAc-(1--> The OPS structure of the strain of E. albertii O7 studied was identical to that of strain LMG 20973 (= Albert 10457), whose structure has been reported earlier (R. Eserstam et al. Eur. J. Biochem. 269 (2002) 3289-3295). E. albertii O3 and O6 shared the OPS structures with Escherichia coli O181 and O3, respectively, except for the lack of O-acetylation in E. albertii O3, which is present in E. coli O181. The gene clusters driving the O-antigen biosynthesis of the E. albertii strains were sequenced, the genes were annotated by comparison with sequences in the available databases, and the predicted functions of the encoded proteins were found to be consistent with the OPS structures established. In accordance with the relatedness of the OPS structures, the O-antigen gene clusters of E. albertii O3 and O6 contain the same genes and have the same organization as those of E. coli O181 and O3, the entire gene clusters being 83% and 98% identical, respectively.