dbCAN-PUL

PUL ID	PUL0462
PubMed	24607538, Carbohydr Res. 2014 Mar 31;388:30-6. doi: 10.1016/j.carres.2014.02.013. Epub 2014 Feb 18.
Characterization method	sequence homology analysis,sugar utilization assay,NMR
Genomic accession number	JHHJ01000126.1
Nucelotide position range	9119-22534
Substrate	O-antigen
Loci	P411_03280-P411_03345
Species	Escherichia coli/562
Degradation or Biosynthesis	biosynthesis

PUL ID	PUL0462
PubMed	24607538, Carbohydr Res. 2014 Mar 31;388:30-6. doi: 10.1016/j.carres.2014.02.013. Epub 2014 Feb 18.
Title	Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages.
Author	Perepelov AV, Filatov AV, Wang Q, L'vov VL, Qian Y, Shashkov AS, Wang L, Knirel YA
Abstract	O-Polysaccharide (O-antigen) accompanied by a minor mannan was isolated from the lipopolysaccharide of Escherichia coli O39 and studied by component analyses, methylation, Smith degradation, mass spectrometry, and 1D and 2D NMR spectroscopy. In addition, a new approach, solvolysis with anhydrous trifluoroacetic acid, was applied to cleave selectively the rhamnosidic linkage. The following structure of the O-polysaccharide was established: alpha--D-Galpl-->3-->3)-beta-D-Quip4N(R3Hb)-(1-->2)-alpha-D-Manp-(l-->4)-alpha-L-Rhap-(1-->3)-alpha-D-GlcpNAc-(1--> where D-Qui4N(R3Hb) indicates 4,6-dideoxy-4-[(R)-3-hydroxybutanoylamino]-d-glucose. The O-antigen gene cluster of E. coli O39 has been sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.