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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0468

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

4457047-4526084

Substrate

host glycan

Loci

BT3461-BT3507

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_3461 AAO78567.1 0 - 435 (+) AE015928.1:4457047-4457482 -
- BT_3462 AAO78568.1 453 - 2208 (+) AE015928.1:4457500-4459255 -
- BT_3463 AAO78569.1 2204 - 2786 (+) AE015928.1:4459251-4459833 -
- BT_3464 AAO78570.1 2782 - 4090 (+) AE015928.1:4459829-4461137 -
- BT_3465 AAO78571.1 4113 - 8151 (+) AE015928.1:4461160-4465198 -
- BT_3466 AAO78572.1 8434 - 8620 (-) AE015928.1:4465481-4465667 -
- BT_3467 AAO78573.1 8740 - 9838 (+) AE015928.1:4465787-4466885 -
- BT_3468 AAO78574.1 9806 - 10670 (-) AE015928.1:4466853-4467717 -
- BT_3469 AAO78575.1 10761 - 12111 (-) AE015928.1:4467808-4469158 -
- BT_3470 AAO78576.1 12169 - 13552 (-) AE015928.1:4469216-4470599 -
- BT_3471 AAO78577.1 13645 - 13888 (-) AE015928.1:4470692-4470935 -
- BT_3472 AAO78578.1 13884 - 15177 (-) AE015928.1:4470931-4472224 -
- BT_3473 AAO78579.1 15278 - 16280 (-) AE015928.1:4472325-4473327 -
- BT_3474 AAO78580.1 16299 - 18345 (-) AE015928.1:4473346-4475392 -
- BT_3475 AAO78581.1 18361 - 21481 (-) AE015928.1:4475408-4478528 -
- BT_3476 AAO78582.1 21505 - 22879 (-) AE015928.1:4478552-4479926 -
- BT_3477 AAO78583.1 22920 - 25443 (-) AE015928.1:4479967-4482490 -
- BT_3478 AAO78584.1 25984 - 27268 (+) AE015928.1:4483031-4484315 -
- BT_3479 AAO78585.1 27712 - 28432 (-) AE015928.1:4484759-4485479 -
- BT_3480 AAO78586.1 28605 - 28989 (-) AE015928.1:4485652-4486036 -
- BT_3481 AAO78587.1 29420 - 29945 (+) AE015928.1:4486467-4486992 -
- BT_3482 AAO78588.1 29962 - 31504 (+) AE015928.1:4487009-4488551 -
- BT_3483 AAO78589.1 31515 - 34656 (+) AE015928.1:4488562-4491703 -
- BT_3484 AAO78590.1 34672 - 36721 (+) AE015928.1:4491719-4493768 -
- BT_3485 AAO78591.1 36737 - 37775 (+) AE015928.1:4493784-4494822 -
- BT_3486 AAO78592.1 37997 - 39359 (+) AE015928.1:4495044-4496406 -
- BT_3487 AAO78593.1 39370 - 41056 (+) AE015928.1:4496417-4498103 -
- BT_3488 AAO78594.1 41084 - 42656 (+) AE015928.1:4498131-4499703 -
- BT_3489 AAO78595.1 42670 - 44047 (+) AE015928.1:4499717-4501094 -
- BT_3490 AAO78596.1 44135 - 45485 (-) AE015928.1:4501182-4502532 -
- BT_3491 AAO78597.1 45919 - 46438 (+) AE015928.1:4502966-4503485 -
- BT_3492 AAO78598.1 46578 - 48063 (+) AE015928.1:4503625-4505110 -
- BT_3493 AAO78599.1 48081 - 48882 (+) AE015928.1:4505128-4505929 -
- BT_3494 AAO78600.1 48881 - 51215 (+) AE015928.1:4505928-4508262 -
- BT_3495 AAO78601.1 51228 - 53295 (+) AE015928.1:4508275-4510342 -
- BT_3496 AAO78602.1 53315 - 54314 (+) AE015928.1:4510362-4511361 -
- BT_3497 AAO78603.1 54397 - 55153 (+) AE015928.1:4511444-4512200 -
- BT_3498 AAO78604.1 55179 - 55851 (+) AE015928.1:4512226-4512898 -
- BT_3499 AAO78605.1 55843 - 56578 (+) AE015928.1:4512890-4513625 -
- BT_3500 AAO78606.1 56710 - 58459 (+) AE015928.1:4513757-4515506 -
- BT_3501 AAO78607.1 58470 - 59949 (+) AE015928.1:4515517-4516996 -
- BT_3502 AAO78608.1 60045 - 60321 (+) AE015928.1:4517092-4517368 -
- BT_3503 AAO78609.1 60552 - 61068 (+) AE015928.1:4517599-4518115 -
- BT_3504 AAO78610.1 61125 - 62682 (+) AE015928.1:4518172-4519729 -
- BT_3505 AAO78611.1 62698 - 65821 (+) AE015928.1:4519745-4522868 -
- BT_3506 AAO78612.1 65836 - 67954 (+) AE015928.1:4522883-4525001 -
- BT_3507 AAO78613.1 67976 - 69038 (+) AE015928.1:4525023-4526085 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 435 (+) CDS No
- 454 - 2208 (+) STP: STP|TPR_2,STP|TPR_1,STP|TPR_2 No
- 2205 - 2786 (+) CDS No
- 2783 - 4090 (+) STP: STP|Peptidase_M23 No
- 4114 - 8151 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
- 8435 - 8620 (-) CDS No
- 8741 - 9838 (+) CAZyme: GH43|GH43_26 Yes
- 9807 - 10670 (-) other Yes
- 10762 - 12111 (-) other Yes
- 12170 - 13552 (-) other Yes
- 13646 - 13888 (-) other Yes
- 13885 - 15177 (-) other Yes
- 15279 - 16280 (-) other Yes
- 16300 - 18345 (-) other Yes
- 18362 - 21481 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 21506 - 22879 (-) other Yes
- 22921 - 25443 (-) other Yes
- 25985 - 27268 (+) other Yes
- 27713 - 28432 (-) other Yes
- 28606 - 28989 (-) other Yes
- 29421 - 29945 (+) other Yes
- 29963 - 31504 (+) other Yes
- 31516 - 34656 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 34673 - 36721 (+) other Yes
- 36738 - 37775 (+) other Yes
- 37998 - 39359 (+) other Yes
- 39371 - 41056 (+) other Yes
- 41085 - 42656 (+) other Yes
- 42671 - 44047 (+) other Yes
- 44136 - 45485 (-) STP: STP|ATPase_2 Yes
- 45920 - 46438 (+) other Yes
- 46579 - 48063 (+) other Yes
- 48082 - 48882 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 48882 - 51215 (+) other Yes
- 51229 - 53295 (+) other Yes
- 53316 - 54314 (+) other Yes
- 54398 - 55153 (+) other Yes
- 55180 - 55851 (+) other Yes
- 55844 - 56578 (+) other Yes
- 56711 - 58459 (+) other Yes
- 58471 - 59949 (+) CAZyme: GH76 Yes
- 60046 - 60321 (+) other Yes
- 60553 - 61068 (+) other Yes
- 61126 - 62682 (+) other Yes
- 62699 - 65821 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 65837 - 67954 (+) CDS No
- 67977 - 69038 (+) CDS No

PUL ID

PUL0468

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.