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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0523

PubMed

20829291, Microbiology (Reading). 2010 Nov;156(Pt 11):3255-3269. doi: 10.1099/mic.0.042978-0. Epub 2010 Sep 9.

Characterization method

sequence homology analysis,microscopy

Genomic accession number

NC_006347.1

Nucelotide position range

3325914-3339788

Substrate

capsule polysaccharide

Loci

BF2921-BF2935

Species

Bacteroides fragilis/817

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BF_RS13895 WP_008777440.1 0 - 300 (+) NC_006347.1:3325914-3326214 -
- BF_RS13900 WP_014299082.1 725 - 1220 (+) NC_006347.1:3326639-3327134 -
- BF_RS13905 WP_011203057.1 1226 - 2345 (+) NC_006347.1:3327140-3328259 -
- BF_RS13910 WP_011203058.1 2350 - 3439 (+) NC_006347.1:3328264-3329353 -
- BF_RS13915 WP_164927417.1 3452 - 4526 (+) NC_006347.1:3329366-3330440 -
- BF_RS13920 WP_011203060.1 4695 - 5415 (+) NC_006347.1:3330609-3331329 -
- BF_RS13925 WP_011203061.1 5424 - 6129 (+) NC_006347.1:3331338-3332043 -
- BF_RS13930 WP_005788655.1 6134 - 7376 (+) NC_006347.1:3332048-3333290 -
- BF_RS13935 WP_008660805.1 7401 - 8688 (+) NC_006347.1:3333315-3334602 -
- BF_RS13940 WP_005815284.1 8727 - 9303 (+) NC_006347.1:3334641-3335217 -
gldL BF_RS13945 WP_005788660.1 9408 - 9921 (+) NC_006347.1:3335322-3335835 -
- BF_RS13950 WP_011203062.1 9932 - 11249 (+) NC_006347.1:3335846-3337163 -
- BF_RS13955 WP_011203063.1 11256 - 12354 (+) NC_006347.1:3337170-3338268 -
- BF_RS13960 WP_005788667.1 12381 - 12810 (+) NC_006347.1:3338295-3338724 -
- BF_RS13965 WP_011203064.1 12825 - 13875 (+) NC_006347.1:3338739-3339789 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 300 (+) CDS No
- 1227 - 2345 (+) CAZyme: GT4 Yes
- 2351 - 3439 (+) CAZyme: GT4 Yes
- 3453 - 4526 (+) CAZyme: GT4 Yes
- 4696 - 5415 (+) CAZyme: GT26 Yes
- 5425 - 6129 (+) other Yes
- 6135 - 7376 (+) other Yes
- 7402 - 8688 (+) other Yes
- 8728 - 9303 (+) other Yes
gldL 9409 - 9921 (+) other Yes
- 9933 - 11249 (+) TC: gnl|TC-DB|Q5EGM3|9.A.25.1.1 Yes
- 11257 - 12354 (+) CDS No
- 12382 - 12810 (+) CDS No
- 12826 - 13875 (+) CDS No

PUL ID

PUL0523

PubMed

20829291, Microbiology (Reading). 2010 Nov;156(Pt 11):3255-3269. doi: 10.1099/mic.0.042978-0. Epub 2010 Sep 9.

Title

Twenty-eight divergent polysaccharide loci specifying within- and amongst-strain capsule diversity in three strains of Bacteroides fragilis.

Author

Patrick S, Blakely GW, Houston S, Moore J, Abratt VR, Bertalan M, Cerdeno-Tarraga AM, Quail MA, Corton N, Corton C, Bignell A, Barron A, Clark L, Bentley SD, Parkhill J

Abstract

Comparison of the complete genome sequence of Bacteroides fragilis 638R, originally isolated in the USA, was made with two previously sequenced strains isolated in the UK (NCTC 9343) and Japan (YCH46). The presence of 10 loci containing genes associated with polysaccharide (PS) biosynthesis, each including a putative Wzx flippase and Wzy polymerase, was confirmed in all three strains, despite a lack of cross-reactivity between NCTC 9343 and 638R surface PS-specific antibodies by immunolabelling and microscopy. Genomic comparisons revealed an exceptional level of PS biosynthesis locus diversity. Of the 10 divergent PS-associated loci apparent in each strain, none is similar between NCTC 9343 and 638R. YCH46 shares one locus with NCTC 9343, confirmed by mAb labelling, and a second different locus with 638R, making a total of 28 divergent PS biosynthesis loci amongst the three strains. The lack of expression of the phase-variable large capsule (LC) in strain 638R, observed in NCTC 9343, is likely to be due to a point mutation that generates a stop codon within a putative initiating glycosyltransferase, necessary for the expression of the LC in NCTC 9343. Other major sequence differences were observed to arise from different numbers and variety of inserted extra-chromosomal elements, in particular prophages. Extensive horizontal gene transfer has occurred within these strains, despite the presence of a significant number of divergent DNA restriction and modification systems that act to prevent acquisition of foreign DNA. The level of amongst-strain diversity in PS biosynthesis loci is unprecedented.