dbCAN-PUL

PUL ID	PUL0603
PubMed	31703861, Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.
Characterization method	microarray,qRT-PCR,culureing methods
Genomic accession number	NC_004567.2
Nucelotide position range	2676726-2679930
Substrate	galactomannooligosaccharide
Loci	LP_RS12650-LP_RS12660
Species	Lactobacillus plantarum WCFS1/1590
Degradation or Biosynthesis	degradation

PUL ID	PUL0603
PubMed	31703861, Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.
Title	Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.
Author	Panwar D, Kapoor M
Abstract	Plant derived galactomannooligosaccharides (GMOS) are an emerging class of prebiotics, but no information is available on their utilization in lactobacilli at the molecular level. The current study aimed at identifying the genetic loci involved in the transport and catabolism of locust bean gum derived GMOS in Lactobacillus plantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degree of polymerization; DP carbohydrate source. Two genetic loci involved in cellobiose (~3.2 kb) and oligo-sucrose (~7.3 kb) utilization in L. plantarum WCFS1 were highly up-regulated up to 8.3 and up to 6.7-fold, respectively by GMOS utilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptome and qRT-PCR studies of L. plantarum WCFS1 suggested that un-substituted mannobiose (DP(2)) might be metabolized by proteins encoded by the cellobiose operon while, substituted DP(2) (galactomannose) and DP(3) (galactomannobiose) were most likely transported and catabolized by the oligo-sucrose utilization loci encoded proteins.