dbCAN-PUL

PUL ID	PUL0606
PubMed	32385941, Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.
Characterization method	enzyme activity assay,clone and expression
Genomic accession number	NC_020517.1
Nucelotide position range	389206-393826
Substrate	beta-galactooligosaccharide
Loci	BBR_RS11735-BBR_RS11745
Species	Bifidobacterium breve UCC2003/1685
Degradation or Biosynthesis	degradation

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 1119 (-)	TF: DBD-Pfam\|LacI,DBD-SUPERFAMILY\|0036955	No
-	1282 - 2727 (-)	TC: gnl\|TC-DB\|A7ZUZ0\|2.A.2.1.1	Yes
-	2801 - 4621 (-)	CAZyme: 3.2.1.23\|GH2	Yes

PUL ID	PUL0606
PubMed	32385941, Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.
Title	Biochemical analysis of cross-feeding behaviour between two common gut commensals when cultivated on plant-derived arabinogalactan.
Author	Munoz J, James K, Bottacini F, Van Sinderen D
Abstract	In this paper, we reveal and characterize cross-feeding behaviour between the common gut commensal Bacteroides cellulosilyticus (Baccell) and certain bifidobacterial strains, including Bifidobacterium breve UCC2003, when grown on a medium containing Larch Wood Arabinogalactan (LW-AG). We furthermore show that cross-feeding is dependent on the release of beta-1,3-galacto-di/trisaccharides (beta-1,3-GOS), and identified that the bga gene cluster of B. breve UCC2003 allows beta-1,3-GOS metabolism. The product of bgaB is presumed to be responsible for the import of beta-1,3-GOS, while the bgaA gene product, a glycoside hydrolase family 2 member, was shown to hydrolyse both beta-1,3-galactobiose and beta-1,3-galactotriose into galactose monomers. This study advances our understanding of strain-specific syntrophic interactions between two glycan degraders in the human gut in the presence of AG-type dietary polysaccharides.