PUL ID

PUL0606

PubMed

32385941, Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.

Characterization method

enzymy activity assay, clone and expression

Genomic accession number

NC_020517.1

Nucelotide position range

389206-393826

Substrate

beta-galactooligosaccharide

Loci

BBR_RS11735-BBR_RS11745

Species

Bifidobacterium breve UCC2003/1685

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BBR_RS11735 WP_015438347.1 0 - 1119 (-) NC_020517.1:389206-390325 -
- BBR_RS11740 WP_015438348.1 1281 - 2727 (-) NC_020517.1:390487-391933 -
- BBR_RS11745 WP_012576707.1 2800 - 4621 (-) NC_020517.1:392006-393827 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1119 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No
- 1282 - 2727 (-) TC: gnl|TC-DB|A7ZUZ0|2.A.2.1.1 Yes
- 2801 - 4621 (-) CAZyme: 3.2.1.23|GH2 Yes

PUL ID

PUL0606

PubMed

32385941, Microb Biotechnol. 2020 Nov;13(6):1733-1747. doi: 10.1111/1751-7915.13577. Epub 2020 May 9.

Title

Biochemical analysis of cross-feeding behaviour between two common gut commensals when cultivated on plant-derived arabinogalactan.

Author

Munoz J, James K, Bottacini F, Van Sinderen D

Abstract

In this paper, we reveal and characterize cross-feeding behaviour between the common gut commensal Bacteroides cellulosilyticus (Baccell) and certain bifidobacterial strains, including Bifidobacterium breve UCC2003, when grown on a medium containing Larch Wood Arabinogalactan (LW-AG). We furthermore show that cross-feeding is dependent on the release of beta-1,3-galacto-di/trisaccharides (beta-1,3-GOS), and identified that the bga gene cluster of B. breve UCC2003 allows beta-1,3-GOS metabolism. The product of bgaB is presumed to be responsible for the import of beta-1,3-GOS, while the bgaA gene product, a glycoside hydrolase family 2 member, was shown to hydrolyse both beta-1,3-galactobiose and beta-1,3-galactotriose into galactose monomers. This study advances our understanding of strain-specific syntrophic interactions between two glycan degraders in the human gut in the presence of AG-type dietary polysaccharides.