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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0609

PubMed

32620774, Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.

Characterization method

enzyme activity assay,clone and expression,liquid chromatography and mass spectrometry,thin layer chromatography,MALDI-TOF/MS

Genomic accession number

NZ_ACFY01000084.1

Nucelotide position range

45466-61920

Substrate

human milk oligosaccharide

Loci

ROSEINA2194_RS08245-ROSEINA2194_RS08295

Species

Roseburia inulinivorans DSM 16841/360807

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

gnpA ROSEINA2194_RS08245 WP_007885595.1 0 - 2169 (-) NZ_ACFY01000084.1:45466-47635 2.4.1.211
- ROSEINA2194_RS08250 WP_044927509.1 2180 - 3353 (-) NZ_ACFY01000084.1:47646-48819 -
- ROSEINA2194_RS21035 WP_007885600.1 3318 - 3492 (-) NZ_ACFY01000084.1:48784-48958 -
- ROSEINA2194_RS08255 WP_007885603.1 3562 - 4405 (-) NZ_ACFY01000084.1:49028-49871 -
- ROSEINA2194_RS21040 - 4437 - 5855 (-) NZ_ACFY01000084.1:49903-51321 -
- ROSEINA2194_RS08265 WP_156337721.1 5902 - 6694 (-) NZ_ACFY01000084.1:51368-52160 -
- ROSEINA2194_RS08270 WP_081453893.1 6772 - 7672 (-) NZ_ACFY01000084.1:52238-53138 -
- ROSEINA2194_RS08275 WP_007885619.1 7765 - 9178 (-) NZ_ACFY01000084.1:53231-54644 -
- ROSEINA2194_RS08280 WP_007885620.1 9369 - 11160 (+) NZ_ACFY01000084.1:54835-56626 -
- ROSEINA2194_RS08285 WP_007885621.1 11161 - 12757 (+) NZ_ACFY01000084.1:56627-58223 -
- ROSEINA2194_RS19690 WP_007885622.1 12837 - 15399 (-) NZ_ACFY01000084.1:58303-60865 -
- ROSEINA2194_RS08295 WP_007885623.1 15432 - 16455 (-) NZ_ACFY01000084.1:60898-61921 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2169 (-) CDS No
- 2181 - 3353 (-) CAZyme: 3.2.1.63|GH95 Yes
- 3319 - 3492 (-) other Yes
- 3563 - 4405 (-) CAZyme: 3.2.1.63|GH95 Yes
- 5903 - 6694 (-) TC: gnl|TC-DB|Q8RJU8|3.A.1.1.18 Yes
- 6773 - 7672 (-) TC: gnl|TC-DB|Q8RJU9|3.A.1.1.18 Yes
- 7766 - 9178 (-) STP: STP|SBP_bac_1 Yes
- 9370 - 11160 (+) TC: gnl|TC-DB|F4LXP4|8.A.59.2.1 Yes
- 11162 - 12757 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 12838 - 15399 (-) CAZyme: GH136|3.2.1.- Yes
- 15433 - 16455 (-) CDS No

PUL ID

PUL0609

PubMed

32620774, Nat Commun. 2020 Jul 3;11(1):3285. doi: 10.1038/s41467-020-17075-x.

Title

Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways.

Author

Pichler MJ, Yamada C, Shuoker B, Alvarez-Silva C, Gotoh A, Leth ML, Schoof E, Katoh T, Sakanaka M, Katayama T, Jin C, Karlsson NG, Arumugam M, Fushinobu S, Abou Hachem M

Abstract

The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support the growth of Roseburia and Eubacterium members on distinct human milk oligosaccharides (HMOs). The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Analyses of 4599 Roseburia genomes underscored the preponderance and diversity of the HMO utilization loci within the genus. The catabolism of HMOs by butyrate-producing Clostridiales may contribute to the competitiveness of this group during the weaning-triggered maturation of the microbiota.