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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0613

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKG010000003.1

Nucelotide position range

315037-327406

Substrate

O-glycan,N-glycan

Loci

HPS57_05940-HPS57_05965

Species

Prevotella sp. PINT/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS57_05940 NPD81511.1 0 - 3108 (+) JABKKG010000003.1:315037-318145 -
- HPS57_05945 NPD81512.1 3126 - 4779 (+) JABKKG010000003.1:318163-319816 -
- HPS57_05950 NPD81513.1 4978 - 5533 (+) JABKKG010000003.1:320015-320570 -
- HPS57_05955 NPD81514.1 5609 - 7967 (+) JABKKG010000003.1:320646-323004 -
- HPS57_05960 - 7983 - 8931 (+) JABKKG010000003.1:323020-323968 -
- HPS57_05965 NPD81515.1 9757 - 12370 (+) JABKKG010000003.1:324794-327407 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3108 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 3127 - 4779 (+) TC: gnl|TC-DB|F9ZC67|8.A.46.1.2 Yes
- 4979 - 5533 (+) other Yes
- 5610 - 7967 (+) CAZyme: PL38| GH88|GH88| PL38 Yes
- 9758 - 12370 (+) CAZyme: CBM57|GH2| GH2| CBM57 Yes

PUL ID

PUL0613

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.