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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0615

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKG010000004.1

Nucelotide position range

347323-358632

Substrate

pectin

Loci

HPS57_07910-HPS57_07935

Species

Prevotella sp. PINT/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS57_07910 NPD81899.1 0 - 1956 (-) JABKKG010000004.1:347323-349279 -
- HPS57_07915 NPD81900.1 1980 - 5139 (-) JABKKG010000004.1:349303-352462 -
pckA HPS57_07920 NPD81901.1 5514 - 7116 (-) JABKKG010000004.1:352837-354439 4.1.1.49
upp HPS57_07925 NPD81902.1 7343 - 8003 (+) JABKKG010000004.1:354666-355326 2.4.2.9
- HPS57_07930 NPD81903.1 8235 - 9636 (-) JABKKG010000004.1:355558-356959 -
- HPS57_07935 NPD81904.1 9951 - 11310 (-) JABKKG010000004.1:357274-358633 -
- HPS57_07940 NPD81905.1 11309 - 11310 (-) JABKKG010000004.1:358632-358633 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1956 (-) CDS No
- 1981 - 5139 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
pckA 5515 - 7116 (-) other Yes
upp 7344 - 8003 (+) STP: STP|Pribosyltran Yes
- 8236 - 9636 (-) TC: gnl|TC-DB|Q9WZS2|2.A.66.1.28 Yes
- 9952 - 11310 (-) CAZyme: GH28| GH28 Yes
- 11310 - 11310 (-) CDS No

PUL ID

PUL0615

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.