Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0627

PubMed

33159946, Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4.

Characterization method

sugar utilization assay,NMR,Smith degradation,mass spectrometry,sequence homology analysis

Genomic accession number

KC526913.2

Nucelotide position range

3089-27646

Substrate

capsule polysaccharide

Loci

None

Species

Acinetobacter baumannii LUH5543/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - AHB32699.1 0 - 2187 (-) KC526913.2:3089-5276 -
wzb - AHB32698.1 2206 - 2635 (-) KC526913.2:5295-5724 -
wza - AHB32697.1 2639 - 3740 (-) KC526913.2:5728-6829 -
gna - AHB32696.1 4095 - 5370 (+) KC526913.2:7184-8459 -
gtr78 - AHB32695.1 5399 - 6263 (+) KC526913.2:8488-9352 -
wzx - AHB32694.2 6262 - 7699 (+) KC526913.2:9351-10788 -
ugd4 - AHB32693.1 7695 - 8859 (+) KC526913.2:10784-11948 -
rmlB - AHB32692.1 8877 - 9945 (+) KC526913.2:11966-13034 -
rmlD - AHB32691.1 9947 - 10841 (+) KC526913.2:13036-13930 -
rmlA - AHB32690.1 10837 - 11728 (+) KC526913.2:13926-14817 -
rmlC - AHB32689.1 11717 - 12269 (+) KC526913.2:14806-15358 -
gtr145 - AHB32688.1 12272 - 13358 (+) KC526913.2:15361-16447 -
wzy - AHB32687.1 13386 - 14625 (+) KC526913.2:16475-17714 -
gtr112 - AHB32686.1 14662 - 15562 (+) KC526913.2:17751-18651 -
gtr82 - AHB32685.1 15561 - 16377 (+) KC526913.2:18650-19466 -
itrA3 - AHB32684.1 16413 - 17016 (+) KC526913.2:19502-20105 -
galU - AHB32683.1 17046 - 17922 (+) KC526913.2:20135-21011 -
ugd - AHB32682.1 17939 - 19202 (+) KC526913.2:21028-22291 -
gpi - AHB32681.1 19198 - 20878 (+) KC526913.2:22287-23967 -
pgt1 - AHB32680.2 21318 - 23160 (+) KC526913.2:24407-26249 -
pgm - AHB32679.1 23187 - 24558 (-) KC526913.2:26276-27647 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2187 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 2207 - 2635 (-) other Yes
- 2640 - 3740 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 4096 - 5370 (+) other Yes
- 5400 - 6263 (+) CAZyme: GT2 Yes
- 6263 - 7699 (+) other Yes
- 7696 - 8859 (+) other Yes
- 8878 - 9945 (+) other Yes
- 9948 - 10841 (+) other Yes
- 10838 - 11728 (+) other Yes
- 11718 - 12269 (+) other Yes
- 12273 - 13358 (+) other Yes
- 13387 - 14625 (+) other Yes
- 14663 - 15562 (+) CAZyme: GT2 Yes
- 15562 - 16377 (+) CAZyme: GT2 Yes
- 16414 - 17016 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 17047 - 17922 (+) CDS No
- 17940 - 19202 (+) CDS No
- 19199 - 20878 (+) CDS No
- 21319 - 23160 (+) CDS No
- 23188 - 24558 (-) CDS No

PUL ID

PUL0627

PubMed

33159946, Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4.

Title

Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85.

Author

Kenyon JJ, Arbatsky NP, Sweeney EL, Zhang Y, Senchenkova SN, Popova AV, Shneider MM, Shashkov AS, Liu B, Hall RM, Knirel YA

Abstract

KL55, KL74, and KL85 capsular polysaccharide (CPS) biosynthesis loci in Acinetobacter baumannii BAL_204, BAL_309, and LUH5543 genomes, respectively, are related and each contains genes for l-Rhap and d-GlcpA synthesis. The CPSs were isolated and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The K55 and K74 CPSs are built up of branched octasaccharide repeats (K units) containing one residue each of d-GlcpA and d-GlcpNAc and six residues of l-Rhap. The K55 unit differs from the K74 unit in the linkage between D-GlcpA and an l-Rhap residue in the K unit (1 --> 3 versus 1 --> 2) and linkage between K units. However, most K units in the isolated K74 CPS were modified by beta-elimination of a side-chain alpha-l-Rhap-(1 --> 3)-alpha-l-Rhap disaccharide from position 4 of GlcA to give 4-deoxy-l-threo-hex-4-enuronic acid (1:~3 ratio of intact and modified units). The K85 CPS has a branched heptasaccharide K unit similar to the K74 unit but with one fewer alpha-l-Rhap residue in the side chain. In contrast to previous findings on A. baumannii CPSs, each K locus includes fewer glycosyltransferase (Gtr) genes than the number required to form all linkages in the K units. Hence, one Gtr appears to be multifunctional catalysing formation of two 1 --> 2 and one 1 --> 3 linkages between the l-Rha residues.