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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0656

PubMed

32236137, PLoS Pathog. 2020 Apr 1;16(4):e1008281. doi: 10.1371/journal.ppat.1008281. eCollection 2020 Apr.

Characterization method

gene deletion mutant and growth assay,complementation study,clone and expression,isothermal titration calorimetry

Genomic accession number

NC_003909.8

Nucelotide position range

5169301-5177236

Substrate

exopolysaccharide

Loci

BCE_RS26735-BCE_RS26760

Species

Bacillus cereus ATCC 10987/1396

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BCE_RS26735 WP_000455000.1 0 - 819 (-) NC_003909.8:5169301-5170120 -
- BCE_RS26740 WP_000559343.1 1109 - 2333 (+) NC_003909.8:5170410-5171634 -
- BCE_RS26745 WP_000601985.1 2332 - 3253 (+) NC_003909.8:5171633-5172554 -
- BCE_RS26750 WP_000715313.1 3236 - 5069 (+) NC_003909.8:5172537-5174370 -
pelF BCE_RS26755 WP_001219499.1 5072 - 6494 (+) NC_003909.8:5174373-5175795 -
pelG BCE_RS26760 WP_000907294.1 6481 - 7936 (+) NC_003909.8:5175782-5177237 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 819 (-) CAZyme: GH0|GH166 Yes
- 1110 - 2333 (+) other Yes
- 2333 - 3253 (+) other Yes
- 3237 - 5069 (+) other Yes
pelF 5073 - 6494 (+) CAZyme: GT4|GT4 Yes
pelG 6482 - 7936 (+) TC: gnl|TC-DB|A3RZV8|2.A.66.6.2 Yes

PUL ID

PUL0656

PubMed

32236137, PLoS Pathog. 2020 Apr 1;16(4):e1008281. doi: 10.1371/journal.ppat.1008281. eCollection 2020 Apr.

Title

Discovery and characterization of a Gram-positive Pel polysaccharide biosynthetic gene cluster.

Author

Whitfield GB, Marmont LS, Bundalovic-Torma C, Razvi E, Roach EJ, Khursigara CM, Parkinson J, Howell PL

Abstract

Our understanding of the biofilm matrix components utilized by Gram-positive bacteria, and the signalling pathways that regulate their production are largely unknown. In a companion study, we developed a computational pipeline for the unbiased identification of homologous bacterial operons and applied this algorithm to the analysis of synthase-dependent exopolysaccharide biosynthetic systems. Here, we explore the finding that many species of Gram-positive bacteria have operons with similarity to the Pseudomonas aeruginosa pel locus. Our characterization of the pelDEADAFG operon from Bacillus cereus ATCC 10987, presented herein, demonstrates that this locus is required for biofilm formation and produces a polysaccharide structurally similar to Pel. We show that the degenerate GGDEF domain of the B. cereus PelD ortholog binds cyclic-3',5'-dimeric guanosine monophosphate (c-di-GMP), and that this binding is required for biofilm formation. Finally, we identify a diguanylate cyclase, CdgF, and a c-di-GMP phosphodiesterase, CdgE, that reciprocally regulate the production of Pel. The discovery of this novel c-di-GMP regulatory circuit significantly contributes to our limited understanding of c-di-GMP signalling in Gram-positive organisms. Furthermore, conservation of the core pelDEADAFG locus amongst many species of bacilli, clostridia, streptococci, and actinobacteria suggests that Pel may be a common biofilm matrix component in many Gram-positive bacteria.