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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0660

PubMed

34073255, Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641.

Characterization method

NMR,Smith degradation

Genomic accession number

MK399430.1

Nucelotide position range

3280-27577

Substrate

capsule polysaccharide

Loci

None

Species

Acinetobacter baumannii 48-1789/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - QBM04806.1 0 - 2196 (-) MK399430.1:3280-5476 -
wzb - QBM04807.1 2217 - 2646 (-) MK399430.1:5497-5926 -
wza - QBM04808.1 2647 - 3748 (-) MK399430.1:5927-7028 -
gna - QBM04783.1 3952 - 5230 (+) MK399430.1:7232-8510 -
rmlB - QBM04784.1 5252 - 6329 (+) MK399430.1:8532-9609 -
rmlD - QBM04785.1 6344 - 7250 (+) MK399430.1:9624-10530 -
rmlA - QBM04786.1 7249 - 8143 (+) MK399430.1:10529-11423 -
rmlC - QBM04787.1 8200 - 8764 (+) MK399430.1:11480-12044 -
wzx - QBM04788.1 8763 - 10290 (+) MK399430.1:12043-13570 -
wzy - QBM04789.1 10366 - 11401 (+) MK399430.1:13646-14681 -
gtr27 - QBM04790.1 11387 - 12491 (+) MK399430.1:14667-15771 -
gtr60 - QBM04791.1 12469 - 13270 (+) MK399430.1:15749-16550 -
atr8 - QBM04792.1 13266 - 13863 (+) MK399430.1:16546-17143 -
tle - QBM04793.1 13855 - 14992 (+) MK399430.1:17135-18272 -
gtr29 - QBM04794.1 14992 - 16033 (+) MK399430.1:18272-19313 -
itrA3 - QBM04795.1 16325 - 16940 (+) MK399430.1:19605-20220 -
galU - QBM04796.1 16963 - 17839 (+) MK399430.1:20243-21119 -
ugd - QBM04797.1 17954 - 19217 (+) MK399430.1:21234-22497 -
gpi - QBM04798.1 19213 - 20884 (+) MK399430.1:22493-24164 -
pgt1 - QBM04799.1 21058 - 22900 (+) MK399430.1:24338-26180 -
pgm - QBM04809.1 22927 - 24298 (-) MK399430.1:26207-27578 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2196 (-) TC: gnl|TC-DB|Q45409|8.A.3.3.3 Yes
- 2218 - 2646 (-) other Yes
- 2648 - 3748 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 3953 - 5230 (+) other Yes
- 5253 - 6329 (+) other Yes
- 6345 - 7250 (+) other Yes
- 7250 - 8143 (+) other Yes
- 8201 - 8764 (+) other Yes
- 8764 - 10290 (+) other Yes
- 10367 - 11401 (+) other Yes
- 11388 - 12491 (+) CAZyme: GT4| GT4 Yes
- 12470 - 13270 (+) CAZyme: GT2| GT2 Yes
- 13267 - 13863 (+) other Yes
- 13856 - 14992 (+) other Yes
- 14993 - 16033 (+) other Yes
- 16326 - 16940 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 16964 - 17839 (+) CDS No
- 17955 - 19217 (+) CDS No
- 19214 - 20884 (+) CDS No
- 21059 - 22900 (+) CDS No
- 22928 - 24298 (-) CDS No

PUL ID

PUL0660

PubMed

34073255, Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641.

Title

Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides.

Author

Kasimova AA, Arbatsky NP, Tickner J, Kenyon JJ, Hall RM, Shneider MM, Dzhaparova AA, Shashkov AS, Chizhov AO, Popova AV, Knirel YA

Abstract

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1-->3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes alpha l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.