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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0665

PubMed

34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.

Characterization method

UHPLC-MS,RNA-seq,RT-qPCR

Genomic accession number

NC_004663.1

Nucelotide position range

3852033-3868605

Substrate

pectin

Loci

BT_3043-BT_3049

Species

Bacteroides thetaiotaomicron VPI-5482/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS15430 WP_011108604.1 0 - 1587 (-) NC_004663.1:3852033-3853620 -
- BT_RS15435 WP_008767316.1 1838 - 2726 (-) NC_004663.1:3853871-3854759 -
- BT_RS15440 WP_008767317.1 2774 - 4757 (-) NC_004663.1:3854807-3856790 -
- BT_RS15445 WP_011108605.1 4796 - 7892 (-) NC_004663.1:3856829-3859925 -
- BT_RS15450 WP_008767319.1 7914 - 9216 (-) NC_004663.1:3859947-3861249 -
- BT_RS15455 WP_011108606.1 9570 - 12258 (-) NC_004663.1:3861603-3864291 -
- BT_RS15460 WP_011108607.1 12442 - 16573 (+) NC_004663.1:3864475-3868606 2.7.13.3
- BT_RS15465 WP_008767322.1 16541 - 16573 (-) NC_004663.1:3868574-3868606 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1587 (-) CAZyme: GH30_4|GH30_4 Yes
- 1839 - 2726 (-) other Yes
- 2775 - 4757 (-) other Yes
- 4797 - 7892 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 7915 - 9216 (-) other Yes
- 9571 - 12258 (-) other Yes
- 12443 - 16573 (+) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 Yes
- 16542 - 16573 (-) CAZyme: GH18| GH18 Yes

PUL ID

PUL0665

PubMed

34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.

Title

Discrete genetic loci in human gut Bacteroides thetaiotaomicron confer pectin metabolism.

Author

Li M, Li S, Guo X, Guo C, Wang Y, Du Z, Zhang Z, Xie C, Ding K

Abstract

Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.