dbCAN-PUL

PUL ID	PUL0667
PubMed	34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.
Characterization method	UHPLC-MS,RNA-seq,RT-qPCR
Genomic accession number	NC_004663.1
Nucelotide position range	4883107-4887725
Substrate	pectin
Loci	BT_3763-BT_3766
Species	Bacteroides thetaiotaomicron VPI-5482/818
Degradation or Biosynthesis	degradation

PUL ID	PUL0667
PubMed	34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.
Title	Discrete genetic loci in human gut Bacteroides thetaiotaomicron confer pectin metabolism.
Author	Li M, Li S, Guo X, Guo C, Wang Y, Du Z, Zhang Z, Xie C, Ding K
Abstract	Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.