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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0670

PubMed

34537298, Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16.

Characterization method

NMR,Smith degradation,mass spectrometry

Genomic accession number

MK420047.1

Nucelotide position range

3274-30582

Substrate

capsule polysaccharide

Loci

None

Species

Acinetobacter baumannii KZ-1098/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wzc - QEA72093.1 0 - 2196 (-) MK420047.1:3274-5470 -
wzb - QEA72094.1 2217 - 2646 (-) MK420047.1:5491-5920 -
wza - QEA72095.1 2647 - 3829 (-) MK420047.1:5921-7103 -
gna - QEA72096.1 3952 - 5230 (+) MK420047.1:7226-8504 -
rmlB - QEA72097.1 5252 - 6329 (+) MK420047.1:8526-9603 -
rmlD - QEA72098.1 6345 - 7251 (+) MK420047.1:9619-10525 -
rmlA - QEA72099.1 7250 - 8144 (+) MK420047.1:10524-11418 -
rmlC - QEA72100.1 8201 - 8750 (+) MK420047.1:11475-12024 -
wzx - QEA72101.1 8795 - 10085 (+) MK420047.1:12069-13359 -
gtr53 - QEA72102.1 10074 - 10971 (+) MK420047.1:13348-14245 -
gtr54 - QEA72103.1 10987 - 11767 (+) MK420047.1:14261-15041 -
manC - QEA72104.1 11838 - 13296 (+) MK420047.1:15112-16570 -
wzy - QEA72105.1 13304 - 14426 (+) MK420047.1:16578-17700 -
gtr55 - QEA72106.1 14425 - 15487 (+) MK420047.1:17699-18761 -
gtr28 - QEA72107.1 15533 - 16319 (+) MK420047.1:18807-19593 -
atr6 - QEA72108.1 16306 - 16873 (+) MK420047.1:19580-20147 -
tle - QEA72109.1 16872 - 18006 (+) MK420047.1:20146-21280 -
gtr29 - QEA72110.1 18006 - 19047 (+) MK420047.1:21280-22321 -
itrA3 - QEA72111.1 19337 - 19943 (+) MK420047.1:22611-23217 -
galU - QEA72112.1 19974 - 20850 (+) MK420047.1:23248-24124 -
ugd - QEA72113.1 20965 - 22228 (+) MK420047.1:24239-25502 -
gpi - QEA72114.1 22224 - 23895 (+) MK420047.1:25498-27169 -
pgt1 - QEA72115.1 24069 - 25911 (+) MK420047.1:27343-29185 -
pgm - QEA72116.1 25938 - 27309 (-) MK420047.1:29212-30583 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2196 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 2218 - 2646 (-) other Yes
- 2648 - 3829 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 3953 - 5230 (+) other Yes
- 5253 - 6329 (+) other Yes
- 6346 - 7251 (+) other Yes
- 7251 - 8144 (+) other Yes
- 8202 - 8750 (+) other Yes
- 8796 - 10085 (+) other Yes
- 10075 - 10971 (+) CAZyme: GT0 Yes
- 10988 - 11767 (+) other Yes
- 11839 - 13296 (+) other Yes
- 13305 - 14426 (+) other Yes
- 14426 - 15487 (+) CAZyme: GT4 Yes
- 15534 - 16319 (+) CAZyme: GT2| GT2 Yes
- 16307 - 16873 (+) other Yes
- 16873 - 18006 (+) other Yes
- 18007 - 19047 (+) other Yes
- 19338 - 19943 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 19975 - 20850 (+) CDS No
- 20966 - 22228 (+) CDS No
- 22225 - 23895 (+) CDS No
- 24070 - 25911 (+) CDS No
- 25939 - 27309 (-) CDS No

PUL ID

PUL0670

PubMed

34537298, Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16.

Title

The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase.

Author

Kasimova AA, Arbatsky NP, Timoshina OY, Shneider MM, Shashkov AS, Chizhov AO, Popova AV, Hall RM, Kenyon JJ, Knirel YA

Abstract

The KL26 gene cluster responsible for the synthesis of the K26 capsular polysaccharide (CPS) of Acinetobacter baumannii includes rmlBDAC genes for l-rhamnose (l-Rhap) synthesis, tle to generate 6-deoxy-l-talose (l-6dTalp) from l-Rhap, and a manC gene for D-mannose (D-Manp) that is rare in Acinetobacter CPS. K26 CPS material was isolated from A. baumannii isolate KZ-1098, and studied by sugar analysis, Smith degradation, and one and two-dimensional (1)H and (13)C NMR spectroscopy before and after O-deacetylation with aqueous ammonia. The following structure of the branched hexasaccharide repeating unit of the CPS was established: -->2)-beta-D-Manp-1-->4-beta-D-Glcp-1-->3-alpha-L-6dTalp-1-->3-beta-D-GlcpNAc-(1-->3 upward arrow14 vertical lineAcalpha-L-Rhap-2<--1-alpha-D-Glcp The structural depolymerase of phage vB_AbaP_APK26 cleaved selectively the beta-GlcpNAc-(1 --> 2)-alpha-Manp linkage in the K26 CPS formed by Wzy(K26) to give monomer, dimer, and trimer of the CPS repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as (1)H and (13)C NMR spectroscopy. The wzy(K26) gene responsible for this linkage and the manC gene were only found in six A. baumannii genomes carrying KL26 and one carrying the novel KL148 gene cluster, indicating the rare occurrence of beta-GlcpNAc-(1 --> 2)-alpha-Manp in A. baumannii CPS structures. However, K26 shares a beta-d-Glcp-(1 --> 3)-alpha-l-6dTalp-(1 --> 3)-beta-d-GlcpNAc trisaccharide fragment with a group of related A. baumannii CPSs that have varying patterns of acetylation of l-6dTalp.